Ugwuanyi J O, Obeta J A
Department of Microbiology, University of Nigeria, Nsukka.
Int J Food Microbiol. 1991 Jun;13(2):157-64. doi: 10.1016/0168-1605(91)90057-v.
A total of 42 soil samples collected from different parts of Nsukka, Southern Nigeria, and 20 freshly plucked mango fruits were screened for the presence of heat-resistant fungi. Each soil sample, suspended in sterile water, and washings from each mango fruit were separately heated at 70 degrees C for 1 h before plating on double-strength potato dextrose agar containing chloramphenicol and incubating at approx. 28 degrees C. Approximately 98% of all soil samples and 17% of mango fruits contained heat-resistant fungi which were identified as Neosartorya fischeri, N. fischeri var. spinosa, Aspergillus flavus, Penicillium citrinum and Paecilomyces varioti which has not been reported in tropical soil. Neosartorya spp. were predominant and occurred in all positive samples. Fungal counts ranged from non-detectable to 200 colony-forming units per 10 g of soil. Most isolates appeared to grow faster at 35 degrees C than at 30 degrees C and 40 degrees C.
对从尼日利亚南部Nsukka不同地区采集的42份土壤样本以及20个新鲜采摘的芒果果实进行了耐热真菌检测。每个悬浮于无菌水中的土壤样本以及每个芒果果实的冲洗液，在接种到含有氯霉素的双倍强度马铃薯葡萄糖琼脂平板上之前，先分别在70摄氏度下加热1小时，然后在约28摄氏度下培养。大约98%的土壤样本和17%的芒果果实含有耐热真菌，这些真菌被鉴定为费氏新萨托菌、费氏新萨托菌刺变种、黄曲霉、桔青霉和变幻拟青霉，其中变幻拟青霉在热带土壤中尚未见报道。新萨托菌属占主导地位，出现在所有阳性样本中。真菌计数范围为每10克土壤中不可检测到200个菌落形成单位。大多数分离株在35摄氏度下的生长速度似乎比在30摄氏度和40摄氏度下更快。
