van der Torre Mireille H, Whitby Cheryl, Eades Christopher P, Moore Caroline B, Novak-Frazer Lilyann, Richardson Malcolm D, Rautemaa-Richardson Riina
Mycology Reference Centre Manchester, ECMM Centre of Excellence for Medical Mycology, Manchester University NHS Foundation Trust, Wythenshawe Hospital, Manchester M23 9LT, UK.
Division of Infection, Inflammation and Respiratory Medicine, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9NT, UK.
J Fungi (Basel). 2020 Oct 6;6(4):208. doi: 10.3390/jof6040208.
The emergence of azole-resistant (AR) complicates the treatment of aspergillosis and can nearly double the mortality from invasive aspergillosis (IA). AR has been isolated from many different environmental sites and indoor environments and thus presents a significant risk for susceptible patients. Local surveillance of environmental AR can guide antifungal prescribing and improve patient outcomes. In this study, seventy-four soils samples collected from the surface of a variety of root vegetables from farm shops and private gardens covering a wide geographical area of the UK, were cultured to assess the presence of and the prevalence and nature of any resistance mechanisms. A high-throughput in-house antifungal susceptibility screening method was developed and validated using the EUCAST MIC reference method, E.DEF 9.3.1. A total of 146 isolates were recovered and analysed. Even though the study premise was that soil-covered root vegetables and other fresh produce could represent a conduit for AR exposure in vulnerable patients, no AR were found in the soil samples despite 55% of samples harbouring The sample type and screening method used could be suitable for more extensive monitoring of the soil to detect trends in the prevalence of AR
唑类耐药（AR）的出现使曲霉病的治疗变得复杂，并且可使侵袭性曲霉病（IA）的死亡率几乎翻倍。AR已从许多不同的环境场所和室内环境中分离出来，因此对易感患者构成重大风险。对环境中的AR进行局部监测可指导抗真菌药物的处方并改善患者预后。在本研究中，从英国广大地理区域的农贸市场和私人花园中采集了74份来自各种根菜类蔬菜表面的土壤样本，进行培养以评估AR的存在情况以及任何耐药机制的流行情况和性质。使用EUCAST MIC参考方法E.DEF 9.3.1开发并验证了一种高通量的内部抗真菌药敏筛选方法。共分离并分析了146株菌株。尽管该研究的前提是被土壤覆盖的根菜类蔬菜和其他新鲜农产品可能是易感患者接触AR的一个途径，但尽管55%的样本中含有曲霉属真菌，土壤样本中未发现AR。所使用的样本类型和筛选方法可能适用于对土壤进行更广泛的监测，以检测AR流行趋势。
