Baldini F, Bolzoni L, Giannetti A, Kess M, Krämer P M, Kremmer E, Porro G, Senesi F, Trono C
Institute of Applied Physics, CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, FI, Italy.
Anal Bioanal Chem. 2009 Feb;393(4):1183-90. doi: 10.1007/s00216-008-2547-1. Epub 2008 Dec 19.
A new immunosensor for the determination of procalcitonin was developed. A sandwich assay format was implemented on a polymethylmetacrylate optical biochip, opportunely shaped in order to obtain several flow channels and potentially suitable for point of care testing applications. The sandwich format makes use of two new rat monoclonal antibodies. The capture antibody was covalently immobilised on the surface of the plastic chip, and the detection antibody was labelled with DY647 dye. Different combinations of capture and detection antibodies were investigated, and particular attention was devoted in order to avoid the non-specific adsorption. A limit of detection of 0.088 mg L(-1) was achieved within the working range of 0.28-50 mg L(-1) in buffer samples. The assay was also implemented in human serum, and 0.2 and 0.7-25 mg L(-1) were the attained limit of detection and working range, respectively.
开发了一种用于测定降钙素原的新型免疫传感器。在聚甲基丙烯酸甲酯光学生物芯片上采用夹心测定法，该芯片形状经过适当设计，以获得多个流动通道，并且可能适用于即时检测应用。夹心测定法使用两种新型大鼠单克隆抗体。捕获抗体共价固定在塑料芯片表面，检测抗体用DY647染料标记。研究了捕获抗体和检测抗体的不同组合，并特别注意避免非特异性吸附。在缓冲液样品中，在0.28 - 50 mg L(-1)的工作范围内，检测限达到0.088 mg L(-1)。该测定法也在人血清中进行，检测限和工作范围分别达到0.2和0.7 - 25 mg L(-1)。
