Michalová E, Poprach A, Nemecková I, Nenutil R, Valík D, Zaloudík J, Vyzula R, Vojtesek B
Masarykův Onkologický Ustav, Oddelení Onkologicikéa Experimentální Patologie, Brno.
Klin Onkol. 2008;21(3):93-7.
Certain hope is entertained in the prediction of chemosensitivity in vitro/ ex vivo for the purpose of selecting the most effective treatment of malignant diseases with minimal patient loading. The possible choice of an effective substance based on the results of a simple ex vivo test would increase the success of the treatment in case of standard chemotherapy failure or in the treatment of primary chemoresistant tumor. MTT test seems to be an easy process for the prediction of chemosensitivity of isolated malignant cells ex vivo, however each method represents a simple tool, which can provide false results if incorrectly preformed. Numerous limitations significantly reduce the successful evaluation and constituent aspects of the methodic press to further reflections about the proper application of the test.
为了在最小化患者负担的情况下选择治疗恶性疾病的最有效方法，人们对体外/离体化学敏感性预测抱有一定希望。基于简单的离体试验结果选择有效物质，可能会在标准化疗失败或治疗原发性化疗耐药肿瘤的情况下提高治疗成功率。MTT试验似乎是预测离体分离恶性细胞化学敏感性的一个简便过程，然而每种方法都只是一个简单工具，如果操作不当可能会得出错误结果。众多局限性显著降低了该方法成功评估的可能性及其构成要素，促使人们进一步思考该试验的正确应用。
