Freisinger Christina M, Houston Douglas W, Slusarski Diane C
Department of Biology, University of Iowa, Iowa City, USA.
Methods Mol Biol. 2008;468:145-56. doi: 10.1007/978-1-59745-249-6_11.
Many aspects of animal development are dependent on the dynamic release of calcium (Ca2+) ions. Although Ca2+ release within a cell is tightly controlled, how the release dynamics result in a specific biological output in embryonic development is less clear. The integration of pharmacological and molecular-genetic studies with in vivo imaging in zebrafish and Xenopus has linked endogenous Ca2+ release to the Wnt signaling network. Our data suggests that distinct Ca2+ release dynamics lead to antagonism of the developmentally important Wnt/beta-catenin pathway while sustained Ca2+ release modulates polarized cell movements.
动物发育的许多方面都依赖于钙离子（Ca2+）的动态释放。尽管细胞内Ca2+的释放受到严格控制，但在胚胎发育中，释放动力学如何导致特定的生物学输出尚不清楚。药理学和分子遗传学研究与斑马鱼和非洲爪蟾体内成像的结合，已将内源性Ca2+释放与Wnt信号网络联系起来。我们的数据表明，不同的Ca2+释放动力学导致对发育中重要的Wnt/β-连环蛋白途径产生拮抗作用，而持续的Ca2+释放则调节极化细胞运动。
