通过抗lck单克隆抗体MOL 171检测到p60lck在人嗜T淋巴细胞病毒I型（HTLV-I）转化的T细胞系中的积累。
Accumulation of p60lck in HTLV-I-transformed T cell lines detected by an anti-lck monoclonal antibody, MOL 171.
作者信息
Moroi Y, Koga Y, Nakamura K, Ohtsu M, Kimura G, Nomoto K
机构信息
Department of Immunology, Kyushu University, Fukuoka.
出版信息
Jpn J Cancer Res. 1991 Aug;82(8):909-15. doi: 10.1111/j.1349-7006.1991.tb01920.x.
The lck gene encodes a protein tyrosine kinase of nonreceptor type, p56lck, whose expression occurs almost exclusively in T lymphocytes. MOL 171, an anti-p56lck monoclonal antibody, was produced by using a 25-amino-acid synthetic polypeptide as the antigen, its structure corresponding to the N-terminal region deduced from the lck cDNA sequence. Immunoblot analysis with MOL 171 showed the accumulation of 60 kD form of Lck protein, p60lck, and the decrease of p56lck in human T cell leukemia virus type I (HTLV-I)-transformed T cell lines. Another anti-Lck monoclonal antibody, MOL 294, raised by using a synthetic peptide corresponding to the C-terminal region deduced from the lck cDNA sequence, also detected the accumulation of p60lck in those HTLV-I-transformed T cell lines. The appearance of p60lck with the decrease of p56lck in normal T lymphocytes after stimulation suggested the origin of p60lck in HTLV-I-transformed T cells.
lck基因编码一种非受体型蛋白酪氨酸激酶p56lck，其表达几乎仅发生于T淋巴细胞。MOL 171是一种抗p56lck单克隆抗体，通过使用一种25个氨基酸的合成多肽作为抗原制备而成，其结构对应于从lck cDNA序列推导的N端区域。用MOL 171进行的免疫印迹分析显示，在人I型嗜T细胞病毒（HTLV-I）转化的T细胞系中，Lck蛋白的60 kD形式p60lck积累，而p56lck减少。另一种抗Lck单克隆抗体MOL 294是通过使用对应于从lck cDNA序列推导的C端区域的合成肽制备的，它也检测到了那些HTLV-I转化的T细胞系中p60lck的积累。正常T淋巴细胞在刺激后p60lck出现而p56lck减少，这提示了HTLV-I转化的T细胞中p60lck的来源。
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