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面向生态学家的DNA条形码技术

DNA barcoding for ecologists.

作者信息

Valentini Alice, Pompanon François, Taberlet Pierre

机构信息

Laboratoire d'Ecologie Alpine, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France.

出版信息

Trends Ecol Evol. 2009 Feb;24(2):110-7. doi: 10.1016/j.tree.2008.09.011. Epub 2008 Dec 25.

Abstract

DNA barcoding - taxon identification using a standardized DNA region - has received much attention recently, and is being further developed through an international initiative. We anticipate that DNA barcoding techniques will be increasingly used by ecologists. They will be able to not only identify a single species from a specimen or an organism's remains but also determine the species composition of environmental samples. Short DNA fragments persist in the environment and might allow an assessment of local biodiversity from soil or water. Even DNA-based diet composition can be estimated using fecal samples. Here we review the new avenues offered to ecologists by DNA barcoding, particularly in the context of new sequencing technologies.

摘要

DNA条形码技术——利用标准化DNA区域进行分类单元鉴定——近年来备受关注,并正通过一项国际倡议得到进一步发展。我们预计生态学家将越来越多地使用DNA条形码技术。他们不仅能够从标本或生物体残骸中识别单个物种,还能确定环境样本的物种组成。短DNA片段在环境中持续存在,可能有助于从土壤或水中评估当地生物多样性。甚至可以使用粪便样本估计基于DNA的饮食组成。在此,我们回顾DNA条形码技术为生态学家提供的新途径,特别是在新测序技术背景下的新途径。

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