Ma Wenlong, Li Jiawei, Qu Xiaolin, Sun Shao'e, Zhou Yanan, Liu Yitong, Wang Peng, Sha Zhongli
Key Laboratory of Advanced Marine Materials, Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
Institute of Marine Corrosion Protection Guangxi Key Laboratory of Marine Environmental Science Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, 530007, China.
Adv Sci (Weinh). 2025 Jan;12(4):e2408718. doi: 10.1002/advs.202408718. Epub 2024 Dec 4.
DNA barcode detection method is widely applied for species identification, which is imperative to evaluate the effect of human economic activities on the biodiversity of ecosystem. However, the wide utilization of existing detection biosensors is limited by bulky and expensive instruments, such as Raman spectroscopy and electrochemical station. Herein, a liquid-solid triboelectric nanogenerator (TENG)-based DNA barcode detection biosensor is proposed, which consists of water flow, fluid channel, and PDMS film attached by specifically designed capture probe. Through sequentially combining capture probe, targeted DNA barcode, and signal probe with Au nanoparticles (NPs), the surface charge density of friction layer of TENG decreases under the effect of AuNPs, verified by the density functional theory (DFT) method. Consequently, the peak value of output current spike signal for targeted DNA is smaller than that for other DNA, which is the working mechanism of the present TENG-based biosensor. Such biosensor successfully recognizes Alvinocaris muricola among different types of Alvinocarididae shrimps, and its low limit detection can reach 1×10 m. The present work provides a paradigm-shift way to develop an inexpensive and accurate technique to detect DNA barcode for species identification, and paves a novel way for the application of liquid-solid TENG.
DNA条形码检测方法被广泛应用于物种识别，这对于评估人类经济活动对生态系统生物多样性的影响至关重要。然而，现有检测生物传感器的广泛应用受到诸如拉曼光谱仪和电化学工作站等体积庞大且昂贵仪器的限制。在此，提出了一种基于液固摩擦纳米发电机（TENG）的DNA条形码检测生物传感器，它由水流、流体通道以及附着有特定设计捕获探针的聚二甲基硅氧烷（PDMS）薄膜组成。通过依次将捕获探针、靶向DNA条形码和带有金纳米颗粒（NPs）的信号探针相结合，在金纳米颗粒的作用下，TENG摩擦层的表面电荷密度降低，这通过密度泛函理论（DFT）方法得到验证。因此，靶向DNA的输出电流尖峰信号峰值小于其他DNA的，这就是当前基于TENG的生物传感器的工作机制。这种生物传感器成功地在不同类型的深海虾中识别出阿尔文虾属（Alvinocaris）虾类，其低检测限可达1×10⁻⁹m。目前的工作为开发一种用于物种识别的廉价且准确的DNA条形码检测技术提供了一种范式转变的方法，并为液固TENG的应用开辟了一条新途径。
