Mbassa G K, Poulsen J S
Department of Clinical Studies, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Zentralbl Veterinarmed A. 1991 Jun;38(5):350-6. doi: 10.1111/j.1439-0442.1991.tb01021.x.
Dilutions of goat blood with Hayem-Jørgensen's fluid ranging from 1:200 to 1:1,000 were used for haemocytometer counting of red blood cells (RBC) in 27 goats. The optimal dilutions were 1:400-1:500. Correlation studies between the results obtained by the haemocytometer and the Coulter counter red blood cell (RBC) and white blood cell (WBC) counts were performed in 551 goat blood samples. The haemocytometer RBC counts were 5.63% higher and WBC counts 2.79% lower than those of the electronic counter. The method of blood cell counting therefore influences the clinical haematological diagnoses and reference values in domestic animals. New cell counters specifically designed to measure cells of small volumes, e.g. goat erythrocytes, are needed.
用Hayem - 约根森氏液将山羊血液稀释至1:200至1:1,000的范围，用于对27只山羊的红细胞（RBC）进行血细胞计数器计数。最佳稀释度为1:400 - 1:500。对551份山羊血液样本进行了血细胞计数器计数结果与库尔特计数器红细胞（RBC）和白细胞（WBC）计数之间的相关性研究。血细胞计数器的RBC计数比电子计数器高5.63%，WBC计数比电子计数器低2.79%。因此，血细胞计数方法会影响家畜的临床血液学诊断和参考值。需要专门设计用于测量小体积细胞（如山羊红细胞）的新型细胞计数器。
