按照修改后的暂行NCCLS程序对新型血液学细胞计数仪——Cell-Dyn 3000进行的简短评估。

A short evaluation of a new haematological cell counter--the Cell-Dyn 3000--following a modified tentative NCCLS-procedure.

作者信息

van Leeuwen L, Eggels P H, Bullen J A

机构信息

Medische Laboratoria, Sint Jansgasthuis, Weert, The Netherlands.

出版信息

Eur J Clin Chem Clin Biochem. 1991 Feb;29(2):105-10. doi: 10.1515/cclm.1991.29.2.105.

DOI:10.1515/cclm.1991.29.2.105

PMID:2049478

Abstract

The proposed procedure offers a method of evaluation that is feasible, but still a very precise method, for the evaluation of electronic differential leukocyte counters. Following this abbreviated method, the classification of the leukocytes, from 5 x 20 unselected blood specimens, with a flow-cytometric analysis by the Sequoia Turner Cell-Dyn 3000, was compared with microscopic differential counts as the standard. With proven accuracy and good reproducibility, the Cell-Dyn 3000 is a good sieve for the selection of normal samples. The high sensitivity and fair specificity permits a reduction of the differential workload to about 30 percent of the normal workload.

摘要

所提出的程序提供了一种可行但仍非常精确的评估方法，用于评估电子白细胞分类计数器。按照这种简化方法，使用Sequoia Turner Cell-Dyn 3000进行流式细胞分析，对5×20份未筛选的血液标本中的白细胞进行分类，并与作为标准的显微镜分类计数进行比较。Cell-Dyn 3000具有经证实的准确性和良好的可重复性，是筛选正常样本的良好工具。其高灵敏度和相当高的特异性可将分类工作量减少至正常工作量的约30%。