Bearinger Jane P, Stone Gary, Hiddessen Amy L, Dugan Lawrence C, Wu Ligang, Hailey Philip, Conway James W, Kuenzler Tobias, Feller Lydia, Cerritelli Simona, Hubbell Jeffrey A
Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory L-211, 7000 East Avenue, Livermore, California, USA.
Langmuir. 2009 Jan 20;25(2):1238-44. doi: 10.1021/la802727s.
Photocatalytic lithography (PCL) is an inexpensive, fast, and robust method of oxidizing surface chemical moieties to produce patterned substrates. This technique has utility in basic biological research as well as various biochip applications. We report on porphyrin-based PCL for patterning poly(propylene sulfide) block copolymer films on gold substrates on the micrometer and submicrometer scales. We confirm chemical patterning with imaging ToF-SIMS and low-voltage SEM. Biomolecular patterning on micrometer and submicrometer scales is demonstrated with proteins, protein-linked beads. and fluorescently labeled proteins.
光催化光刻（PCL）是一种廉价、快速且稳健的方法，可用于氧化表面化学基团以制备图案化基板。该技术在基础生物学研究以及各种生物芯片应用中均有实用价值。我们报道了基于卟啉的PCL技术，用于在微米和亚微米尺度上在金基板上制备聚（硫化丙烯）嵌段共聚物薄膜的图案。我们通过成像飞行时间二次离子质谱（ToF-SIMS）和低电压扫描电子显微镜（SEM）确认了化学图案。利用蛋白质、蛋白质连接的珠子和荧光标记的蛋白质，展示了微米和亚微米尺度上的生物分子图案化。
