Christman Karen L, Enriquez-Rios Vanessa D, Maynard Heather D
Department of Chemistry and Biochemistry, 607 Charles E. Young Drive East, Los Angles, CA 90095-1569 and California NanoSystems Institute, University of California Los Angeles, 6722 Boelter Hall, Los Angles, CA 90095-7151.
Department of Chemistry and Biochemistry, 607 Charles E. Young Drive East, Los Angles, CA 90095-1569.
Soft Matter. 2006 Oct 17;2(11):928-939. doi: 10.1039/b611000b.
A variety of techniques have been developed to site-specifically immobilize biomolecules onto surfaces with resolutions below one micron. The ability to pattern proteins and peptides in particular has great potential for applications in biosensors, biomaterials, and tissue engineering. For example, immobilizing proteins at the nanoscale could lead to the development of diagnostic protein nanoarrays, while patterning peptides could lead to greater control over the cell/biomaterial interface. This review discusses the methods that have been reported for patterning proteins and peptides with submicron and nanometer resolutions.
已经开发出多种技术，用于将生物分子以低于一微米的分辨率位点特异性地固定到表面上。特别是对蛋白质和肽进行图案化的能力在生物传感器、生物材料和组织工程领域具有巨大的应用潜力。例如，在纳米尺度上固定蛋白质可能会促成诊断性蛋白质纳米阵列的开发，而对肽进行图案化则可以更好地控制细胞/生物材料界面。本文综述了已报道的用于以亚微米和纳米分辨率对蛋白质和肽进行图案化的方法。
