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本文引用的文献

1
Antimicrobial susceptibility of Neisseria gonorrhoeae strains isolated in 2004-2006 in Bangui, Central African Republic; Yaoundé, Cameroon; Antananarivo, Madagascar; and Ho Chi Minh Ville and Nha Trang, Vietnam.2004年至2006年在中非共和国班吉、喀麦隆雅温得、马达加斯加塔那那利佛以及越南胡志明市和芽庄分离出的淋病奈瑟菌菌株的抗菌药敏性。
Sex Transm Dis. 2008 Nov;35(11):941-5. doi: 10.1097/OLQ.0b013e31818318d8.
2
Genotyping as a tool for antibiotic resistance surveillance of Neisseria gonorrhoeae in New Caledonia: evidence of a novel genotype associated with reduced penicillin susceptibility.基因分型作为新喀里多尼亚淋病奈瑟菌抗生素耐药性监测的工具：一种与青霉素敏感性降低相关的新型基因型的证据。
Antimicrob Agents Chemother. 2008 Sep;52(9):3293-300. doi: 10.1128/AAC.00020-08. Epub 2008 Jun 30.
3
Emerging antimicrobial resistance in Neisseria gonorrhoeae: urgent need to strengthen prevention strategies.淋病奈瑟菌新出现的抗菌药物耐药性：迫切需要加强预防策略。
Ann Intern Med. 2008 Apr 15;148(8):606-13. doi: 10.7326/0003-4819-148-8-200804150-00005.
4
Prediction of antibiotic resistance using Neisseria gonorrhoeae multi-antigen sequence typing.利用淋病奈瑟菌多抗原序列分型预测抗生素耐药性。
Sex Transm Infect. 2008 Aug;84(4):280-4. doi: 10.1136/sti.2008.029694. Epub 2008 Feb 6.
5
Analysis of trends in antimicrobial resistance in Neisseria gonorrhoeae isolated in Australia, 1997 2006.1997 - 2006年澳大利亚分离的淋病奈瑟菌耐药性趋势分析
J Antimicrob Chemother. 2008 Jan;61(1):150-5. doi: 10.1093/jac/dkm434. Epub 2007 Nov 6.
6
Surveillance of antibiotic resistance in Neisseria gonorrhoeae in the WHO Western Pacific Region, 2005.2005年世界卫生组织西太平洋区域淋病奈瑟菌抗生素耐药性监测
Commun Dis Intell Q Rep. 2006;30(4):430-3. doi: 10.33321/cdi.2006.30.40.
7
Real-time PCR assay for detection of quinolone-resistant Neisseria gonorrhoeae in urine samples.用于检测尿液样本中耐喹诺酮淋病奈瑟菌的实时聚合酶链反应检测法。
J Clin Microbiol. 2007 Apr;45(4):1250-4. doi: 10.1128/JCM.01909-06. Epub 2007 Jan 31.
8
A novel real-time duplex PCR assay for detecting penA and ponA genotypes in Neisseria gonorrhoeae: Comparison with phenotypes determined by the E-test.一种用于检测淋病奈瑟菌中penA和ponA基因型的新型实时双链PCR检测方法：与E-test法测定的表型比较
Clin Chem. 2006 Dec;52(12):2294-6. doi: 10.1373/clinchem.2006.075309. Epub 2006 Oct 19.
9
Antibiotic resistance in Neisseria gonorrhoeae is diminishing available treatment options for gonorrhea: some possible remedies.淋病奈瑟菌的抗生素耐药性正在减少淋病可用的治疗选择：一些可能的补救措施。
Expert Rev Anti Infect Ther. 2006 Aug;4(4):619-28. doi: 10.1586/14787210.4.4.619.
10
The worldwide emergence of plasmid-mediated quinolone resistance.质粒介导的喹诺酮耐药性在全球范围内的出现。
Lancet Infect Dis. 2006 Oct;6(10):629-40. doi: 10.1016/S1473-3099(06)70599-0.

淋病奈瑟菌对喹诺酮类药物的耐药性：通过熔解曲线分析对gyrA和parC基因中喹诺酮类耐药决定区进行快速基因分型可预测药敏性。

Quinolone resistance in Neisseria gonorrhoeae: rapid genotyping of quinolone resistance-determining regions in gyrA and parC genes by melting curve analysis predicts susceptibility.

作者信息

Vernel-Pauillac Frédérique, Hogan Tiffany R, Tapsall John W, Goarant Cyrille

机构信息

Laboratoire de Recherche en Bactériologie, Institut Pasteur de Nouvelle-Calédonie, Nouméa Cedex, New Caledonia.

出版信息

Antimicrob Agents Chemother. 2009 Mar;53(3):1264-7. doi: 10.1128/AAC.01104-08. Epub 2009 Jan 5.

DOI:10.1128/AAC.01104-08
PMID:19124663
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2650556/
Abstract

We report a duplex real-time PCR assay for the simultaneous screening of mutations involved in fluoroquinolone resistance within gyrA and parC quninolone resistance-determining regions (QRDRs) in Neisseria gonorrhoeae. Our assay clearly detects all mutated QRDRs and allows the identification of common genotypes, whether the QRDRs contain single or double mutations, providing valuable epidemiological tools. When this method is used in conjunction with similar assays and in vitro analyses, essential antibiotic resistance surveillance can be performed for public health purposes.

摘要

我们报告了一种双重实时PCR检测方法，用于同时筛查淋病奈瑟菌中gyrA和parC喹诺酮耐药决定区（QRDRs）内与氟喹诺酮耐药相关的突变。我们的检测方法能够清晰地检测到所有突变的QRDRs，并能够识别常见的基因型，无论QRDRs包含单突变还是双突变，从而提供有价值的流行病学工具。当该方法与类似检测方法及体外分析结合使用时，可为公共卫生目的进行必要的抗生素耐药监测。