淋病奈瑟菌对喹诺酮类药物的耐药性：通过熔解曲线分析对gyrA和parC基因中喹诺酮类耐药决定区进行快速基因分型可预测药敏性。
Quinolone resistance in Neisseria gonorrhoeae: rapid genotyping of quinolone resistance-determining regions in gyrA and parC genes by melting curve analysis predicts susceptibility.
作者信息
Vernel-Pauillac Frédérique, Hogan Tiffany R, Tapsall John W, Goarant Cyrille
机构信息
Laboratoire de Recherche en Bactériologie, Institut Pasteur de Nouvelle-Calédonie, Nouméa Cedex, New Caledonia.
出版信息
Antimicrob Agents Chemother. 2009 Mar;53(3):1264-7. doi: 10.1128/AAC.01104-08. Epub 2009 Jan 5.
Abstract
We report a duplex real-time PCR assay for the simultaneous screening of mutations involved in fluoroquinolone resistance within gyrA and parC quninolone resistance-determining regions (QRDRs) in Neisseria gonorrhoeae. Our assay clearly detects all mutated QRDRs and allows the identification of common genotypes, whether the QRDRs contain single or double mutations, providing valuable epidemiological tools. When this method is used in conjunction with similar assays and in vitro analyses, essential antibiotic resistance surveillance can be performed for public health purposes.
摘要
我们报告了一种双重实时PCR检测方法，用于同时筛查淋病奈瑟菌中gyrA和parC喹诺酮耐药决定区（QRDRs）内与氟喹诺酮耐药相关的突变。我们的检测方法能够清晰地检测到所有突变的QRDRs，并能够识别常见的基因型，无论QRDRs包含单突变还是双突变，从而提供有价值的流行病学工具。当该方法与类似检测方法及体外分析结合使用时，可为公共卫生目的进行必要的抗生素耐药监测。
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