Ermertcan Aylin Türel, Inan Sevinc, Ozturkcan Serap, Bilac Cemal, Cilaker Serap
Department of Dermatology, Celal Bayar University, Faculty of Medicine, Manisa, Turkey.
Wound Repair Regen. 2008 Sep-Oct;16(5):674-81. doi: 10.1111/j.1524-475X.2008.00417.x.
In this study, we compared the effects of collagenase and Centella asiatica in the rat model. Twenty-seven female rats were divided into three groups, and two full-thickness wounds were made for each animal. Collagenase ointment was applied topically to Group I and C. asiatica ointment to Group II rats. In Group III, no treatment was applied. On the third day of treatment, wounds on the left side of three animals of each group were excised. On the fifth and eighth day of the treatments, the same procedure was performed for the remaining animals. Indirect immunohistochemical examination was performed to detect transforming growth factor beta (TGF)-beta, endothelial and inducible nitric oxide synthase (eNOS and iNOS), vascular endothelial growth factor, TGF-alpha, laminin, fibronectin, collagen I, and interleukin-1beta. According to the measurements of the wound areas and wound healing periodo, collagenase was superior to the control group. Immunohistochemical examinations showed strong (+++) iNOS and TGF-beta immunoreactivities in C. asiatica group. eNOS immunoreactivity was moderate (++) in this group. For the collagenase group, iNOS, eNOS, and TGF-beta immunoreactivities were moderate (++). In the collagenase group, while TGF-beta and iNOS immunoreactivities were weaker, laminin and fibronectin reactivities were stronger than in C. asiatica and control groups. Collagenase was superior to C. asiatica according to the immunohistochemical findings. Collagenase ointment significantly improves the quality of wound healing and scar formation and is a more appropriate treatment choice than extract of C. asiatica in the early stages of the wound healing process.
在本研究中，我们在大鼠模型中比较了胶原酶和积雪草的作用。将27只雌性大鼠分为三组，每只动物制作两个全层伤口。给第一组大鼠局部应用胶原酶软膏，给第二组大鼠局部应用积雪草软膏。第三组不进行治疗。在治疗的第三天，切除每组三只动物左侧的伤口。在治疗的第五天和第八天，对其余动物进行相同操作。进行间接免疫组织化学检查以检测转化生长因子β（TGF）-β、内皮型和诱导型一氧化氮合酶（eNOS和iNOS）、血管内皮生长因子、TGF-α、层粘连蛋白、纤连蛋白、I型胶原和白细胞介素-1β。根据伤口面积和伤口愈合期的测量结果，胶原酶优于对照组。免疫组织化学检查显示，积雪草组iNOS和TGF-β免疫反应性强（+++）。该组eNOS免疫反应性中等（++）。对于胶原酶组，iNOS、eNOS和TGF-β免疫反应性中等（++）。在胶原酶组中，虽然TGF-β和iNOS免疫反应性较弱，但层粘连蛋白和纤连蛋白反应性比积雪草组和对照组更强。根据免疫组织化学结果，胶原酶优于积雪草。胶原酶软膏可显著改善伤口愈合质量和瘢痕形成，在伤口愈合过程的早期阶段是比积雪草提取物更合适的治疗选择。
