Kuo Chi-Wen, Chiu Yi-Fang, Wu Min-Hua, Li Ming-Hsien, Wu Cheng-Nan, Chen Wan-Sin, Huang Chiung-Hua
Department of Pharmacy, Jen-Ai Hospital No. 483, Dong Rong Rd., Tali, Taichung 412, Taiwan.
Department of Nursing, College of Nursing, Central Taiwan University of Science and Technology, 666 Buzi Rd., Beitun District, Taichung City 406, Taiwan.
Polymers (Basel). 2021 Feb 15;13(4):579. doi: 10.3390/polym13040579.
Acne is a chronic inflammatory skin disease that often occurs with anaerobic (). Anti-acne patches, made of hydrocolloid or hydrogel, have become a popular way of topical treatment. The outer water-impermeable layer of commercial patches might create hypoxic conditions and promote growth. In this study, gelatin/chitosan (GC) bilayer patches were prepared at different temperatures that included room temperature (RT), -20 °C/RT, and -80 °C/RT. The most promising GC bilayer patch (-80 °C /RT) contained a dense upper layer for protection from bacteria and infection and a porous lower layer for absorbing pus and fluids from pimples. The anti-acne bilayer patch was loaded with Cortex (PA) and (CA) extracts. PA extract could inhibit the growth of and CA extract was reported to improve wound healing and reduce scar formation. Moreover, the water retention rate, weight loss rate, antibacterial activity, and in vitro cytotoxicity of the patches were investigated. The porous structure of the patches promoted water retention and contributed to absorbing the exudate when used on open acne wounds. The GC bilayer patches loaded with PA/CA extracts were demonstrated to inhibit the growth of , and accelerate the skin fibroblast cell viability. Based on their activities and characteristics, the GC bilayer patches with PA/CA extract prepared at -80 °C/RT obtain the potential for the application of acne spot treatment.
痤疮是一种慢性炎症性皮肤病，常与厌氧菌（）共同发生。由水胶体或水凝胶制成的抗痤疮贴片已成为一种流行的局部治疗方法。商业贴片的外部不透水层可能会造成缺氧条件并促进（此处原文缺失相关内容）生长。在本研究中，在不同温度下制备了明胶/壳聚糖（GC）双层贴片，包括室温（RT）、-20°C/RT和-80°C/RT。最有前景的GC双层贴片（-80°C/RT）包含一个致密的上层用于防止细菌和感染，以及一个多孔的下层用于吸收粉刺中的脓液和液体。抗痤疮双层贴片负载了黄柏（PA）和儿茶（CA）提取物。PA提取物可抑制（此处原文缺失相关内容）的生长，据报道CA提取物可促进伤口愈合并减少疤痕形成。此外，还研究了贴片的保水率、失重率、抗菌活性和体外细胞毒性。贴片的多孔结构促进了保水，并在用于开放性痤疮伤口时有助于吸收渗出物。负载PA/CA提取物的GC双层贴片被证明可抑制（此处原文缺失相关内容）的生长，并加速皮肤成纤维细胞的活力。基于其活性和特性，在-80°C/RT下制备的负载PA/CA提取物的GC双层贴片具有用于痤疮点治疗的应用潜力。
