Pehlivan Sacide, Ozkinay Ferda, Alper Sibel, Onay Huseyin, Yuksel Eda, Pehlivan Mustafa, Ozkinay Cihangir
Gaziantep University Faculty of Medicine, Department of Medical Biology, 27060 Gaziantep, Turkey.
Eur J Dermatol. 2009 Mar-Apr;19(2):126-8. doi: 10.1684/ejd.2008.0578. Epub 2009 Jan 7.
Vitiligo is a common skin disorder characterized by patterned depigmentation, because of a decrease of melanin pigment resulting from apparent melanocyte loss. The aim of this study was to investigate interleukin 4 (IL4), Angiotensin Converting Enzyme (ACE), C-C Chemocine Receptor 5 (CCR5), Cytotoxic T Lymphocyte-associated Antigen Receptor 4 (CTLA4) and Interleukin 1 Receptor Antagonist (IL1-RN) gene polymorphisms in 48 Turkish vitiligo patients and 50 healthy controls. Polymorphisms for the genes ACE insertion(I)/deletion(D), CCR5 (Delta32), IL1-RN (VNTR in intron 2) were detected by PCR methods. IL4 (-590) and CTLA4 (+49) gene polymorphisms were typed using PCR-RFLP methods. No significant differences in either the genotype distribution or allele frequencies of IL4, CCR5 and ACE gene polymorphisms were observed. GG genotype and G allele in CTLA4 genes were found to be significantly higher in vitiligo patients compared to the controls. (0.002, 0.000). CTLA4 (AA) and IL1-RN (1/5) genotypes and 5 allele frequency in the IL1-RN gene were found to be significantly lower in vitiligo patients compared to healthy controls (p: 0.014, 0.015, 0.016, respectively). As a conclusion, CTLA4 and IL1-RN genes might play roles in the genetic etiology of vitiligo.
白癜风是一种常见的皮肤疾病，其特征为出现图案状色素脱失，这是由于明显的黑素细胞丢失导致黑色素减少所致。本研究的目的是调查48例土耳其白癜风患者和50例健康对照者中白细胞介素4（IL4）、血管紧张素转换酶（ACE）、C-C趋化因子受体5（CCR5）、细胞毒性T淋巴细胞相关抗原受体4（CTLA4）和白细胞介素1受体拮抗剂（IL1-RN）基因的多态性。通过PCR方法检测ACE基因插入（I）/缺失（D）、CCR5（Delta32）、IL1-RN（内含子2中的VNTR）基因的多态性。使用PCR-RFLP方法对IL4（-590）和CTLA4（+49）基因多态性进行分型。未观察到IL4、CCR5和ACE基因多态性的基因型分布或等位基因频率有显著差异。与对照组相比，白癜风患者CTLA4基因的GG基因型和G等位基因明显更高（分别为0.002，0.000）。与健康对照组相比，白癜风患者CTLA4（AA）和IL1-RN（1/5）基因型以及IL1-RN基因中的5等位基因频率明显更低（p值分别为0.014、0.015、0.016）。结论是，CTLA4和IL1-RN基因可能在白癜风遗传病因中起作用。
