Tsubura E, Yamashita T, Kobayashi M, Higuchi Y
Gan. 1976 Dec;67(6):849-56.
The inhibitory effect of sulfated polysaccharides on blood-borne metastasis was examined. As a model of blood-borne metastasis, the ascitic form of hepatoma AH-109A tumor was injected intravenously into Donryu strain rats. Examination of the pulmonary metastatic nodules developed 2 weeks later showed inhibitory effect of the five sulfated polysaccharides tested. Xylan sulfate was the most inhibitory, and exerted its inhibitory effect when the tumor cells were in the pulmonary capillary beds. However, fromthe rapid disappearance of radioactivity from the lungs after injection of 125IUDR-labeled AH-109A cells, tumor cells seemed to be retained in the lungs for only a very short time. Measurement of the anticoagulative and fibrinolytic activities of three sulfated polysaccharides showed that the inhibitory effect of these compounds on blood-borne metastasis was proportional to their anticoagulative and fibrinolytic activities, xylan sulfate showing the highest activities. These results suggest that sulfated polyaccharides may inhibit blood-borne pulmonary metastasis by inhibiting the lodging of tumor cells in the pulmonary capillary beds.
研究了硫酸化多糖对血行转移的抑制作用。以血行转移为模型，将腹水型肝癌AH-109A肿瘤静脉注射到东乡株大鼠体内。对2周后形成的肺转移结节进行检查，结果显示所测试的5种硫酸化多糖均有抑制作用。硫酸木聚糖的抑制作用最强，且在肿瘤细胞处于肺毛细血管床时发挥抑制作用。然而，注射125IUDR标记的AH-109A细胞后，肺部放射性迅速消失，这表明肿瘤细胞在肺部仅停留很短时间。对3种硫酸化多糖的抗凝和纤溶活性进行测定，结果表明这些化合物对血行转移的抑制作用与其抗凝和纤溶活性成正比，硫酸木聚糖的活性最高。这些结果表明，硫酸化多糖可能通过抑制肿瘤细胞在肺毛细血管床的滞留来抑制血行性肺转移。
