Azaiez Aïda, Boyle Brian, Levée Valérie, Séguin Armand
Natural Resources Canada, Canadien Forest Service, Laurentian Forestry Centre, Sainte-Foy, Quebec, Canada.
Mol Plant Microbe Interact. 2009 Feb;22(2):190-200. doi: 10.1094/MPMI-22-2-0190.
In natural conditions, plants are subjected to a combination of biotic stresses and often have to cope with simultaneous pathogen infections. In this report, we aim to understand the global transcriptional response of hybrid poplar NM6 (Populus nigra x P. maximowiczii) to infection by two biotrophic Melampsora fungi, Melampsora larici-populina and M. medusae f. sp. deltoidae. These pathogens triggered different responses after inoculation of poplar leaves. Transcript profiling using the GeneChip Poplar Genome Array revealed a total of 416 differentially expressed transcripts whose expression level was > or = twofold relative to controls. Interestingly, approximately half of the differentially expressed genes in infected leaves showed altered expression following interaction with either of the Melampsora spp. We also infected poplar leaves simultaneously with both Melampsora spp. to investigate potential interaction between the responses to the individual pathogens during a mixed infection. For this mixed inoculation, the number of differentially expressed transcripts increased to 648 and our analysis showed that infection with both fungi also induced a common set of genes. The genes induced after Melampsora spp. infection were mainly related to primary and secondary metabolic processes, cell-wall reinforcement and lignification, defense and stress-related mechanisms, and signal perception and transduction.
在自然条件下，植物会遭受多种生物胁迫，常常需要应对同时发生的病原体感染。在本报告中，我们旨在了解杂交杨树NM6（黑杨×大青杨）对两种活体营养型美孢锈菌（落叶松-杨栅锈菌和杨扇舟蛾锈菌变种）感染的整体转录反应。接种杨树叶片后，这些病原体引发了不同的反应。使用基因芯片杨树基因组阵列进行转录谱分析，结果显示共有416个差异表达转录本，其表达水平相对于对照提高了两倍或更高。有趣的是，感染叶片中约一半的差异表达基因在与任何一种美孢锈菌相互作用后表达都发生了改变。我们还同时用两种美孢锈菌感染杨树叶片，以研究混合感染期间对单个病原体的反应之间的潜在相互作用。对于这种混合接种，差异表达转录本的数量增加到648个，我们的分析表明，两种真菌感染还诱导了一组共同的基因。美孢锈菌感染后诱导的基因主要与初级和次级代谢过程、细胞壁强化和木质化、防御和应激相关机制以及信号感知和转导有关。
