木质素代谢涉及到番茄中葡萄孢菌 BcGs1 诱导的防御反应。

Lignin metabolism involves Botrytis cinerea BcGs1- induced defense response in tomato.

机构信息

The State Key Laboratory for Biology of Plant Disease and Insect Pests/ Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture Institute of Plant protection, Chinese Academy of Agricultural science, No. 12 Zhong-guan-cun South Street, Beijing, 100081, China.

出版信息

BMC Plant Biol. 2018 Jun 4;18(1):103. doi: 10.1186/s12870-018-1319-0.

BACKGROUND

BcGs1, a cell wall-degrading enzyme (CWDE), was originally derived from Botrytis cinerea. Our previous study revealed that BcGs1 could trigger defense responses and protect plants against various pathogens. We researched the defense response mechanism underlying this BcGs1 elicitation in tomato.

RESULTS

We revealed that the two domains were required for BcGs1's full necrosis activity. According to analysis and quantitative real-time PCR of the up-regulated proteins and genes filtered by iTRAQ-based quantitative proteome approach, oxidative metabolism and phenylpropanoid metabolism were speculated to be involved in BcGs1-triggered defense response in tomato. Furthermore, experimental evidence showed that BcGs1 triggered reactive oxygen species (ROS) burst and increased the level of phenylalanine-ammonia lyase (PAL) and peroxidase (POD) enzyme activity, as well as lignin accumulation. Moreover, histochemical analysis revealed that infiltration of BcGs1 in tomato leaves exhibited cell wall thickening compared with untreated plants.

CONCLUSIONS

The results suggested that BcGs1 activated the basal defense response included lignin metabolism contributed to BcGs1-induced resistance to Botrytis. cinerea infection in tomato.

背景

Bgcs1 是一种细胞壁降解酶（CWDE），最初来源于 Botrytis cinerea。我们之前的研究表明，Bgcs1 可以引发防御反应，保护植物免受各种病原体的侵害。我们研究了番茄中这种 BcGs1 激发的防御反应的机制。

结果

我们揭示了两个结构域对于 BcGs1 完全坏死活性是必需的。根据 iTRAQ 定量蛋白质组学方法筛选的上调蛋白和基因的分析和定量实时 PCR，推测氧化代谢和苯丙烷代谢参与了 BcGs1 诱导的番茄防御反应。此外，实验证据表明，Bgcs1 触发了活性氧（ROS）爆发，增加了苯丙氨酸解氨酶（PAL）和过氧化物酶（POD）酶活性以及木质素积累的水平。此外，组织化学分析显示，Bgcs1 在番茄叶片中的渗透与未处理的植物相比表现出细胞壁增厚。