Gryczynski I, Matveeva E G, Sarkar P, Bharill S, Borejdo J, Mandecki W, Akopova I, Gryczynski Z
Center for Commercialization of Fluorescence Technologies, Dept. of Molecular Biology and Immunology, UNTHSC, Fort Worth, TX 76107.
Chem Phys Lett. 2008 Oct;462(4-6):327-330. doi: 10.1016/j.cplett.2008.08.008.
We report on the fluorescence enhancement induced by silver island film (SIF) deposited on a silicon wafer. The model immunoassay was studied on silvered and unsilvered wafers. The fluorescence brightness of Rhodamine Red X increased about 300% on the SIF, while the lifetime was reduced by several fold and the photostability increased substantially. We discuss potential uses of silicon wafer substrates in multiplex assays in which the fluorescence is enhanced due to the SIF, and the multiplexing is achieved by using micro transponders.
我们报道了沉积在硅片上的银岛膜(SIF)所引起的荧光增强现象。在镀银和未镀银的硅片上研究了模型免疫测定。若丹明红X在银岛膜上的荧光亮度增加了约300%,而其寿命缩短了几倍,并且光稳定性大幅提高。我们讨论了硅片基底在多重分析中的潜在用途,在这种分析中,由于银岛膜荧光得以增强,并且通过使用微型应答器实现多重分析。