Sasamoto K, Zenko R, Ueno K, Ohkura Y
Dojindo Laboratories, Kumamoto, Japan.
Chem Pharm Bull (Tokyo). 1991 May;39(5):1317-9. doi: 10.1248/cpb.39.1317.
The chemiluminescence assay of urinary N-acetyl-beta-D-glucosaminidase using o-aminophthalylhydrazido-N-acetyl-beta-D-glucosaminide as the substrate was examined. Under optimized conditions, a linear chemiluminescence response to the standard N-acetyl-beta-D-glucosaminidase (NAGase) of different concentrations was observed with a correlation coefficient of 0.999 over the range of 0.3 to 20 I.U./l. The present method was compared with the prevalent method using p-nitrophenyl-N-acetyl-beta-D-glucosaminide in the assay of 20 urine samples from healthy subjects, and the correlation coefficient of 0.891 was obtained.
以邻氨基苯二甲酰肼基 - N - 乙酰 - β - D - 氨基葡萄糖苷为底物，对尿N - 乙酰 - β - D - 氨基葡萄糖苷酶的化学发光测定法进行了研究。在优化条件下，观察到不同浓度的标准N - 乙酰 - β - D - 氨基葡萄糖苷酶（NAGase）呈现线性化学发光响应，在0.3至20 I.U./l范围内相关系数为0.999。将本方法与在检测20例健康受试者尿液样本中使用对硝基苯基 - N - 乙酰 - β - D - 氨基葡萄糖苷的常用方法进行比较，得到的相关系数为0.891。
