Vaubourdolle M, Gufflet V, Chazouillères O, Giboudeau J, Poupon R
Service de Biochimie A, Hôpital Saint Antoine, Paris, France.
Clin Chem. 1991 Oct;37(10 Pt 1):1688-90.
Primary biliary cirrhosis (PBC) is a chronic cholestatic disease in which there is a crucial need for quantitative liver-function tests. We have developed a mixed sulfobromophthalein (BSP)-indocyanine green (ICG) test and have applied it to 15 healthy subjects and 50 patients with PBC to determine its relevance to the histological severity of the disease. The two dyes were administered intravenously and sequentially as boluses. Plasma concentrations were measured over 60 min. Pharmacokinetic analysis of the plasma elimination curve permitted the calculation of clearance, constants k1 and k2, and the retention percentage at 45 min. In PBC patients, ICG kinetics were within the normal range except for those with stage IV disease (cirrhosis). BSP clearance and the k2 constant were reduced in all the patients, whereas the k1 constant was reduced only in stage III and IV disease. The BSP retention percentage at 45 min was highly correlated with histological stage (r = 0.89, P less than 0.001). The BSP-ICG mixed test may thus prove useful in the diagnosis and follow-up of patients with PBC.
原发性胆汁性肝硬化（PBC）是一种慢性胆汁淤积性疾病，对肝功能定量检测有迫切需求。我们研发了一种磺溴酞钠（BSP）-吲哚菁绿（ICG）混合试验，并将其应用于15名健康受试者和50名PBC患者，以确定其与疾病组织学严重程度的相关性。两种染料均以静脉推注的方式依次给药。在60分钟内测量血浆浓度。通过对血浆消除曲线进行药代动力学分析，可以计算清除率、常数k1和k2以及45分钟时的潴留百分比。在PBC患者中，除了IV期疾病（肝硬化）患者外，ICG动力学均在正常范围内。所有患者的BSP清除率和k2常数均降低，而k1常数仅在III期和IV期疾病患者中降低。45分钟时的BSP潴留百分比与组织学分期高度相关（r = 0.89，P < 0.001）。因此，BSP-ICG混合试验可能在PBC患者的诊断和随访中证明有用。
