Connolly Bernard A
Institute for Cell and Molecular Biosciences (ICaMB), University of Newcastle, Newcastle upon Tyne NE2 4HH, UK.
Biochem Soc Trans. 2009 Feb;37(Pt 1):65-8. doi: 10.1042/BST0370065.
Archaeal family-B DNA polymerases interact specifically with uracil and hypoxanthine, stalling replication on encountering these deaminated bases in DNA template strands. The present review describes X-ray structural data which elucidate the mechanism of read-ahead recognition of uracil and suggests how this is coupled to cessation of polymerization. The possible role of read-ahead recognition of uracil/hypoxanthine in DNA repair is discussed, as is the observation that the feature appears to be limited to replicative polymerases of the archaeal domain.
古菌家族B型DNA聚合酶与尿嘧啶和次黄嘌呤特异性相互作用，当在DNA模板链中遇到这些脱氨基碱基时会使复制停滞。本综述描述了X射线结构数据，这些数据阐明了对尿嘧啶的提前识别机制，并提出了其与聚合反应停止的关联方式。文中讨论了尿嘧啶/次黄嘌呤的提前识别在DNA修复中的可能作用，以及该特征似乎仅限于古菌域复制性聚合酶的这一观察结果。
