大鼠胫骨缺损用多孔颗粒状生物陶瓷材料的体内生物相容性及置换动力学研究。

Study of in vivo biocompatibility and dynamics of replacement of rat shin defect with porous granulated bioceramic materials.

作者信息

Chissov V I, Sviridova I K, Sergeeva N S, Frank G A, Kirsanova V A, Achmedova S A, Reshetov I V, Filjushin M M, Barinov S M, Fadeeva I V, Komlev V S

机构信息

P. A. Hertzen Moscow Oncological Institute, Rosmedtechnologies, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2008 Jul;146(1):139-43. doi: 10.1007/s10517-008-0222-3.

DOI:10.1007/s10517-008-0222-3

PMID:19145372

Abstract

Biocompatibility of porous granulated bioceramic materials (hydroxyapatite, beta-tricalcium phosphate, hydroxyapatite-b-tricalcium phosphate complex (80:20 wt%), carbonate-containing hydroxyapatite, and silicon-containing hydroxyapatite) was shown in a subcutaneous test on BDF1 mice. Dynamic (up to 8 months) observation showed gradual replacement of the granular substance with de novo forming bone tissue with hemopoiesis foci on a model of fenestral defect in the shin bone in Wistar rats. By the rate of resorption, the materials rank as follows: silicon-containing hydroxyapatite<hydroxyapatite<hydroxyapatite-beta-tricalcium phosphate<beta-tricalcium phosphate<carbonate-containing hydroxyapatite. The rate of resorption in bone tissue defect was significantly higher than in the subcutaneous test, but lagged behind (even for tricalcium phosphate and carbonate-containing hydroxyapatite) bone tissue formation de novo.

摘要

在BDF1小鼠的皮下试验中显示了多孔粒状生物陶瓷材料（羟基磷灰石、β-磷酸三钙、羟基磷灰石-β-磷酸三钙复合物（80:20重量%）、含碳酸羟基磷灰石和含硅羟基磷灰石）的生物相容性。动态（长达8个月）观察表明，在Wistar大鼠胫骨开窗缺损模型中，颗粒物质逐渐被新生的骨组织替代，且有造血灶。就吸收速率而言，材料排序如下：含硅羟基磷灰石＜羟基磷灰石＜羟基磷灰石-β-磷酸三钙＜β-磷酸三钙＜含碳酸羟基磷灰石。骨组织缺损处的吸收速率明显高于皮下试验，但（即使对于磷酸三钙和含碳酸羟基磷灰石）仍落后于新生骨组织的形成。