Fuh Lih-Jyh, Huang Ya-Jing, Chen Wen-Cheng, Lin Dan-Jae
School of Dentistry, College of Medicine, China Medical University, Taichung 404, Taiwan, ROC; Department of Dentistry, Section of Prosthodontics, China Medical University Hospital, Taichung 404, Taiwan, ROC.
School of Dentistry, College of Medicine, China Medical University, Taichung 404, Taiwan, ROC.
Mater Sci Eng C Mater Biol Appl. 2017 Jun 1;75:798-806. doi: 10.1016/j.msec.2017.02.065. Epub 2017 Feb 21.
Dimensional instability caused by sintering shrinkage is an inevitable drawback for conventional processing of hydroxyapatite (HA). A new preparation method for biphasic calcium phosphates was developed to increase micro pores and biodegradation without significant dimensional change. Powder pressed HA discs, under 100MPa, were immersed in a colloidal mixture of tetraethoxysilane (TEOS) and ammonium hydroxide for 10min, followed by drying, and then were sintered at 900°C, 1050°C, and 1200°C, respectively. Comparing with pure HA discs, the newly prepared product sintered up to 1200°C contained silicon substituted HA, beta-tricalcium phosphate, and calcium silicate with better micro-porosity, high specific surface area, less sintering shrinkage and the strength maintained. The cytocompatibility test demonstrated a better viability for D1 mice stem cells cultured on TEOS treated HA for 14days compared to the pure HA. This simple TEOS sol-gel pretreatment has the potential to be applied to any existing manufacturing process of HA scaffold for better control of sintering shrinkage, create micropores, and increase biodegradation.
烧结收缩引起的尺寸不稳定性是羟基磷灰石（HA）传统加工中不可避免的缺点。开发了一种新的双相磷酸钙制备方法，以增加微孔和生物降解性，同时尺寸变化不显著。在100MPa压力下压制的HA圆盘，浸入四乙氧基硅烷（TEOS）和氢氧化铵的胶体混合物中10分钟，然后干燥，接着分别在900°C、1050°C和1200°C下烧结。与纯HA圆盘相比，新制备的在1200°C烧结的产品含有硅取代的HA、β-磷酸三钙和硅酸钙，具有更好的微孔性、高比表面积、更小的烧结收缩率且强度得以保持。细胞相容性测试表明，与纯HA相比，在经TEOS处理的HA上培养14天的D1小鼠干细胞具有更好的活力。这种简单的TEOS溶胶-凝胶预处理有潜力应用于HA支架的任何现有制造工艺，以更好地控制烧结收缩、产生微孔并增加生物降解性。
