Lerebours Adélaïde, Gonzalez Patrice, Adam Christelle, Camilleri Virginie, Bourdineaud Jean-Paul, Garnier-Laplace Jacqueline
Laboratoire de Radioécologie et d'Ecotoxicologie, Institut de Radioprotection et de Sûreté Nucléaire, Bât 183, BP 3, 13115 Saint-Paul-Lez-Durance Cedex, France.
Environ Toxicol Chem. 2009 Jun;28(6):1271-8. doi: 10.1897/08-357.1.
The effects of waterborne uranium (U) exposure on gene expression were examined in four organs (brain, liver, skeletal muscles, and gills) of the zebrafish (Danio rerio). Adult male fish were exposed to three treatments: No added uranium (control), 23 +/- 6 microg U/L, and 130 +/- 34 microg U/L. After 3, 10, 21, and 28 d of exposure and an 8-d depuration period, gene expression and uranium bioaccumulation were analyzed. Bioaccumulation decreased significantly in liver during the depuration phase, and genes involved in detoxification, apoptotic mechanism, and immune response were strongly induced. Among these genes, abcb311, which belongs to the adenosine triphosphate (ATP)-binding cassette transporter family, was induced 4- and 24-fold in organisms previously exposed to 23 +/- 6 and 130 +/- 34 microg U/L, respectively. These results highlight the role of liver in detoxification mechanisms. In gills, at the highest uranium concentration, gpx1a, cat1, sod1, and sod2 genes were up-regulated at day 21, indicating the onset of an oxidative stress. Mitochondrial metabolism and DNA integrity also were affected, because coxI, atp5f1, and rad51 genes were up-regulated at day 21 and during the depuration phase. In skeletal muscles, coxI, atp5f1, and cat were induced at day 3, suggesting an impact on the mitochondrial metabolism and production of reactive oxygen species. In brain, glsI also was induced at day 3, suggesting a need in the glutamate synthesis involved with neuron transmission. No changes in gene expression were observed in brain and skeletal muscles at days 21 and 28, although bioaccumulation increased. During the depuration phase, uranium excretion was inefficient in brain and skeletal muscles, and expression of most of the tissue-specific genes was repressed or unchanged.
研究了斑马鱼（Danio rerio）暴露于水中铀（U）对其四个器官（脑、肝、骨骼肌和鳃）基因表达的影响。成年雄鱼接受三种处理：不添加铀（对照）、23±6微克U/L和130±34微克U/L。在暴露3、10、21和28天后以及8天的净化期后，分析基因表达和铀生物累积情况。在净化阶段，肝脏中的生物累积显著下降，参与解毒、凋亡机制和免疫反应的基因被强烈诱导。在这些基因中，属于三磷酸腺苷（ATP）结合盒转运蛋白家族的abcb311，在先前暴露于23±6和130±34微克U/L的生物体中分别被诱导4倍和24倍。这些结果突出了肝脏在解毒机制中的作用。在鳃中，在最高铀浓度下，gpx1a、cat1、sod1和sod2基因在第21天上调，表明氧化应激开始。线粒体代谢和DNA完整性也受到影响，因为coxI、atp5f1和rad51基因在第21天和净化阶段上调。在骨骼肌中，coxI、atp5f1和cat在第3天被诱导，表明对线粒体代谢和活性氧产生有影响。在脑中，glsI在第3天也被诱导，表明在与神经元传递相关的谷氨酸合成中有需求。在第21天和28天，脑和骨骼肌中未观察到基因表达变化，尽管生物累积增加。在净化阶段，脑和骨骼肌中的铀排泄效率低下，大多数组织特异性基因的表达被抑制或未改变。
