大孔树脂法纯化复方雪莲滴丸中羌活与独活的工艺研究
[Purification technology of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis in Fufang Xuelian dropping pills by macroporous resin].
作者信息
Luo Yu-Qin, Yang Wei-Jun, Xu Jian-Guo, Xing Jian-Guo
机构信息
Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Nov;33(21):2478-81.
OBJECTIVE
To study the purification technology of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis in Fufang Xuelian dropping pills by macroporous resin.
METHOD
Taking osthole, isomperatorin as index ingredients, the type of resin sampling amount and elution solvent were decided, and the influence of sample concentration pH of sample and ratio of diameter to height of column to adsorption were studied.
RESULT
HPD400A was chosen to purify, the suitable sampling ratio of resin volume to raw material was 1:2; pH 3.5 (crude drug) and ratio of diameter to height was 1:7; 95% ethanol of the elution solvent was satisfactory eluant for desorption.
CONCLUSION
HPD400A macroporous resin can be used to purify Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis.
目的
研究大孔树脂对复方雪莲滴丸中羌活和独活的纯化工艺。
方法
以蛇床子素、异欧前胡素为指标成分，确定树脂类型、上样量及洗脱溶剂，考察样品浓度、样品pH值及柱径高比对吸附的影响。
结果
选择HPD400A进行纯化，树脂体积与原料的适宜上样比为1∶2；pH值为3.5（粗药），柱径高比为1∶7；洗脱溶剂95%乙醇为满意的解吸剂。
结论
HPD400A大孔树脂可用于羌活和独活的纯化。
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