Zhang Anding, Chen Bo, Mu Xiaofeng, Li Ran, Zheng Pei, Zhao Yaxin, Chen Huanchun, Jin Meilin
Unit of Animal Infectious Diseases, National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei 430070, PR China.
Vaccine. 2009 Feb 25;27(9):1348-53. doi: 10.1016/j.vaccine.2008.12.047. Epub 2009 Jan 15.
Streptococcus suis serotype 2 (SS2) is a porcine and human pathogen with adhesive and invasive properties. The absence of suitable vaccine or virulent marker can be the bottleneck to control SS2 infection. In the present study, a novel immunogenic Enolase identified in the previous study was inducibly overexpressed in Escherichia coli, and the purified recombinant protein could elicit a significant humoral antibody response and confer efficient immunity against challenge with lethal dose of SS2 or SS7 infection in mouse model. The roles Enolase plays in pathogenicity of SS2 were also explored as reasons for which Enolase could be a protective antigen. The Enolase was an in vivo-induced antigen confirmed by the real-time PCR and could adhere to the Hep-2 cells by the indirect immunofluorescent assay and the inhibition assay. These suggested that Enolase could play important roles in pathogenicity and may serve as a novel vaccine candidate against SS2 infection.
猪链球菌2型（SS2）是一种具有黏附性和侵袭性的猪和人病原体。缺乏合适的疫苗或毒力标记可能成为控制SS2感染的瓶颈。在本研究中，前期研究鉴定出的一种新型免疫原性烯醇化酶在大肠杆菌中可诱导过表达，纯化的重组蛋白能引发显著的体液抗体反应，并在小鼠模型中对致死剂量的SS2或SS7感染攻击提供有效免疫。还探讨了烯醇化酶在SS2致病性中所起的作用，以此作为烯醇化酶可成为保护性抗原的原因。通过实时PCR证实烯醇化酶是一种体内诱导抗原，并且通过间接免疫荧光试验和抑制试验表明其可黏附于Hep-2细胞。这些结果表明烯醇化酶在致病性中可能发挥重要作用，并可能作为一种抗SS2感染的新型疫苗候选物。
