野生型和h-ns突变体细胞中StpA的差异结合图谱：通过染色质免疫沉淀-微阵列分析对协同伙伴进行比较分析。
Differential binding profiles of StpA in wild-type and h-ns mutant cells: a comparative analysis of cooperative partners by chromatin immunoprecipitation-microarray analysis.
作者信息
Uyar Ebru, Kurokawa Ken, Yoshimura Mika, Ishikawa Shu, Ogasawara Naotake, Oshima Taku
机构信息
Graduate School of Information Science, Nara Institute of Science and Technology, Japan.
出版信息
J Bacteriol. 2009 Apr;191(7):2388-91. doi: 10.1128/JB.01594-08. Epub 2009 Jan 16.
Abstract
We performed chromatin immunoprecipitation-microarray analysis to investigate differences in function between StpA and H-NS in Escherichia coli cells. StpA binding regions essentially overlap those of H-NS in wild-type cells, while they are reduced to one-third in the hns mutant. The H-NS binding profile was unaffected by stpA inactivation.
摘要
我们进行了染色质免疫沉淀-微阵列分析，以研究大肠杆菌细胞中StpA和H-NS之间的功能差异。在野生型细胞中，StpA结合区域与H-NS的基本重叠，而在hns突变体中，它们减少到三分之一。H-NS的结合图谱不受stpA失活的影响。
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