Noom Maarten C, Navarre William W, Oshima Taku, Wuite Gijs J L, Dame Remus T
Curr Biol. 2007 Nov 6;17(21):R913-4. doi: 10.1016/j.cub.2007.09.005.
The bacterial chromosome is organized into loops, which constitute topologically isolated domains. It is unclear which proteins are responsible for the formation of the topological barriers between domains. The abundant DNA-binding histone-like nucleoid structuring protein (H-NS) is a key player in the organization and compaction of bacterial chromosomes [1,2]. The protein acts by bridging DNA duplexes [3], thus allowing for the formation of DNA loops. Here, genome-wide studies of H-NS binding suggest that this protein is directly involved in the formation or maintenance of topological domain barriers.
细菌染色体被组织成环状结构，这些环状结构构成了拓扑学上隔离的结构域。目前尚不清楚哪些蛋白质负责结构域之间拓扑学屏障的形成。丰富的DNA结合类组蛋白核仁结构蛋白（H-NS）是细菌染色体组织和压缩过程中的关键参与者[1,2]。该蛋白通过桥接DNA双链来发挥作用[3]，从而促进DNA环的形成。在这里，对H-NS结合的全基因组研究表明，这种蛋白质直接参与拓扑结构域屏障的形成或维持。
