Montesinos J J, Flores-Figueroa E, Castillo-Medina S, Flores-Guzmán P, Hernández-Estévez E, Fajardo-Orduña G, Orozco S, Mayani H
Oncology Hospital, National Medical Center, IMSS, Mexico City, Mexico.
Cytotherapy. 2009;11(2):163-76. doi: 10.1080/14653240802582075.
Bone marrow (BM) has been recognized as the main source of mesenchymal stromal cells (MSC); however, MSC have also been detected in umbilical cord blood (UCB) and placenta (PL). In the present study, we obtained MSC from these three sources and characterized them in a comparative manner.
MSC were obtained from BM, UCB and PL samples and analyzed to determine their morphology, cell-surface antigen (Ag) expression and differentiation potential. Particular emphasis was placed on the expression of neural markers.
MSC were detected in 9/9, 11/104 and 5/5 samples from BM, UCB and PL, respectively. MSC populations comprised several morphologically distinct cell types, including neural-like cells. MSC were positive for 'mesenchymal' Ag (CD105, CD73 and CD90), although CD90 expression was very heterogeneous. Interestingly, CD13 expression was high in all three sources. In all cases, MSC showed osteogenic and chondrogenic differentiation; however, UCB MSC showed no adipogenic potential. Furthermore, MSC from UCB produced a different type of cartilage compared with MSC from BM and PL. It is noteworthy that in all three sources we detected the expression of neural proteins without any neural differentiation stimuli. A significant increase in the proportion of neural marker-positive MSC was observed in the presence of neural inducers.
Our results indicate that PL may prove to be a more appropriate source for obtaining MSC than UCB, and suggest the possibility that a subpopulation of MSC may possess neural potential, which is favored by neural inducers.
骨髓（BM）已被公认为间充质基质细胞（MSC）的主要来源；然而，在脐带血（UCB）和胎盘（PL）中也检测到了MSC。在本研究中，我们从这三种来源获取了MSC，并对其进行了比较分析。
从BM、UCB和PL样本中获取MSC，并分析其形态、细胞表面抗原（Ag）表达和分化潜能。特别关注神经标志物的表达。
分别在来自BM、UCB和PL的9/9、11/104和5/5样本中检测到MSC。MSC群体包含几种形态上不同的细胞类型，包括神经样细胞。MSC对“间充质”抗原（CD105、CD73和CD90）呈阳性，尽管CD90表达非常不均一。有趣的是，CD13在所有三种来源中表达都很高。在所有情况下，MSC都表现出成骨和成软骨分化；然而，UCB MSC没有脂肪生成潜能。此外，与来自BM和PL的MSC相比，UCB的MSC产生了不同类型的软骨。值得注意的是，在所有三种来源中，我们在没有任何神经分化刺激的情况下检测到了神经蛋白的表达。在存在神经诱导剂的情况下，观察到神经标志物阳性MSC的比例显著增加。
我们的结果表明，PL可能被证明是比UCB更合适的获取MSC的来源，并提示MSC的一个亚群可能具有神经潜能，神经诱导剂有利于这种潜能的发挥。
