Rosales Velázquez César Alexis, Chavéz Gómez Laura Guadalupe, Félix Espinosa Carlos Arturo, Moreno-Eutimio Mario Adan, Montesinos Juan José, Fajardo-Orduña Guadalupe R, Tirado Mendoza Rocio
Laboratorio de Biología del Citoesqueleto y Virología, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Circuito Interior sin Número, Coyoacán 04510, Mexico.
Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, Edificio A, Circuito Escolar sin Número, Coyoacán 04510, Mexico.
Viruses. 2025 May 28;17(6):763. doi: 10.3390/v17060763.
Acute respiratory tract infections (ARIs) are one of the major causes of morbimortality in children and adulthood. Furthermore, the respiratory syncytial virus (RSV) is the main pathogen in severe lower respiratory tract infections. In Mexico, RSV is the second cause of ARI, affecting mainly children and seniors. RSV infects the airway epithelium, including mesenchymal stem cells (MSCs). These cells express a variety of surface molecules which may function as viral receptors, i.e., Toll-like receptors (TLRs), but the consequences that viral infection has on their biological activities are poorly understood. The aim of this study is to determinate if RSV infection of MSC modifies the expression of stemness biomarkers, TLRs, and the organization of the cytoskeleton. To study the viral infection of MSCs, we determined the mRNA expression using qRT-PCR of SOX2, NANOG, and POU5F1; vimentin and actin; and TLRs 2, 4, and 6. In addition, we determined the cell surface expression of TLR 2 and 4 using flow cytometry. Our results showed that the infection did not change the mRNA expression of SOX2, NANOG, and POU5F1, but increased the mRNA expression of TLR4 and the cell surface expression. Meanwhile, the mRNA in the actin was unchanged, vimentin decreased, and the infection generated a redistribution of the cytoskeleton.
急性呼吸道感染（ARIs）是儿童和成人发病和死亡的主要原因之一。此外，呼吸道合胞病毒（RSV）是严重下呼吸道感染的主要病原体。在墨西哥，RSV是ARI的第二大病因，主要影响儿童和老年人。RSV感染气道上皮，包括间充质干细胞（MSCs）。这些细胞表达多种可能作为病毒受体的表面分子，即Toll样受体（TLRs），但病毒感染对其生物学活性的影响尚不清楚。本研究的目的是确定MSCs感染RSV是否会改变干性生物标志物、TLRs的表达以及细胞骨架的组织。为了研究MSCs的病毒感染，我们使用qRT-PCR测定了SOX2、NANOG和POU5F1；波形蛋白和肌动蛋白；以及TLRs 2、4和6的mRNA表达。此外，我们使用流式细胞术测定了TLR 2和4的细胞表面表达。我们的结果表明，感染并未改变SOX2、NANOG和POU5F1的mRNA表达，但增加了TLR4的mRNA表达和细胞表面表达。同时，肌动蛋白的mRNA未发生变化，波形蛋白减少，并且感染导致细胞骨架重新分布。
