两种不同培养液中胚胎质量和着床率：ISM1 与通用 IVF 培养液。
Embryo quality and implantation rate in two different culture media: ISM1 versus Universal IVF Medium.
机构信息
Mother-Infant Department, Institute of Obstetrics and Gynaecology, University of Modena and Reggio Emilia, Modena, Italy.
出版信息
Fertil Steril. 2010 Apr;93(6):1859-63. doi: 10.1016/j.fertnstert.2008.12.030. Epub 2009 Jan 18.
OBJECTIVE
To compare the outcome of two different culture media marketed by the MediCult AS Company (Jyllinge, Denmark)-Universal IVF Medium and ISM1 Medium culture-which, in addition to glucose, pyruvate, and energy-providing components, also contain amino acids, nucleotides, vitamins, and cholesterol.
DESIGN
Laboratory and retrospective clinical study.
SETTING
University teaching hospital.
PATIENT(S): A total of 726 patients, undergoing IVF-intracytoplasmic sperm injection procedure, comparable in mean age range, oocyte retrieval, and infertility indication, were included in the study. Laboratory quality and standard procedures were maintained unaffected.
INTERVENTION(S): Oocyte retrieval, different embryo culture media.
MAIN OUTCOME MEASURE(S): Embryo quality, ongoing pregnancy, and implantation rate.
RESULT(S): The frequency of good-quality embryos (79% vs. 74%) and the percentages of ongoing pregnancy (27.5% vs. 18%) and implantation rate (15% vs. 10%) were significantly higher in the group treated with ISM1 Medium rather than Universal IVF Medium.
CONCLUSION(S): ISM1 Medium culture seems to improve the performance of embryonic growth and development, as well as increasing the percentage of pregnancy.
目的
比较 MediCult AS 公司（丹麦 Jyllinge）生产的两种不同培养基（通用 IVF 培养基和 ISM1 培养基）的培养效果。这两种培养基除了葡萄糖、丙酮酸和提供能量的成分外，还含有氨基酸、核苷酸、维生素和胆固醇。
设计
实验室和回顾性临床研究。
地点
大学教学医院。
患者
共纳入 726 名接受 IVF-胞浆内单精子注射程序的患者，这些患者的平均年龄范围、卵母细胞采集和不孕原因相似。实验室质量和标准程序保持不变。
干预措施
卵母细胞采集，不同的胚胎培养培养基。
主要观察指标
胚胎质量、持续妊娠和着床率。
结果
与使用通用 IVF 培养基相比，使用 ISM1 培养基的患者中优质胚胎的出现频率（79%比 74%）、持续妊娠率（27.5%比 18%）和着床率（15%比 10%）显著提高。
结论
ISM1 培养基培养似乎可以改善胚胎的生长和发育性能，提高妊娠率。
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