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蓝色玉米:淀粉颗粒的形态及淀粉合酶特性

Blue maize: morphology and starch synthase characterization of starch granule.

作者信息

Utrilla-Coello Rubi G, Agama-Acevedo Edith, de la Rosa Ana Paulina Barba, Martinez-Salgado Jose L, Rodriguez-Ambriz Sandra L, Bello-Perez Luis A

机构信息

Centro de Desarrollo de Productos Bióticos del IPN., Km 8.5 carr Yautepec-Jojutla, colonia San Isidro, P.O. Box 24, 62731, Yautepec, Morelos, Mexico.

出版信息

Plant Foods Hum Nutr. 2009 Mar;64(1):18-24. doi: 10.1007/s11130-008-0106-8.

Abstract

The use of pigmented maize varieties has increased due to their high anthocyanins content, but very few studies are reported about the starch properties of these grains. The aim of this work was to isolate the starch granules from pigmented blue maize and carry out the morphological, physicochemical, and biochemical characterization studies. The proximate composition of starch granules showed high protein contents, after purification, the blue maize starch presented lower protein amount than starch from white maize (control). Although the purity of starch granules was increased, the damaged starch (determined for the Maltase cross absence) was also increased. Scanning electron microscopy showed the presence of some pores and channels in the blue maize starch. The electrophoretic protein profiles showed differences in the bands that correspond to the enzymes involved in the starch biosynthesis; these differences could explain the variation in morphological characteristics of blue maize starches against starch from white maize.

摘要

由于色素玉米品种花青素含量高,其使用量有所增加,但关于这些谷物淀粉特性的研究报道很少。本研究的目的是从色素蓝色玉米中分离淀粉颗粒,并进行形态学、物理化学和生物化学特性研究。淀粉颗粒的近似成分显示蛋白质含量高,纯化后,蓝色玉米淀粉的蛋白含量低于白色玉米(对照)淀粉。虽然淀粉颗粒的纯度提高了,但破损淀粉(通过麦芽糖酶交叉缺失测定)也增加了。扫描电子显微镜显示蓝色玉米淀粉中存在一些孔隙和通道。电泳蛋白质谱显示,与淀粉生物合成相关的酶对应的条带存在差异;这些差异可以解释蓝色玉米淀粉与白色玉米淀粉形态特征的差异。

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