Tripp Joanna, Mishra Shravan Kumar, Scharf Klaus-Dieter
J. W. Goethe-University, Molecular Cell Biology of Plants, Biocenter N200, 3OG, Max-von-Laue-Str. 9, D-60438 Frankfurt, Germany.
Plant Cell Environ. 2009 Feb;32(2):123-33. doi: 10.1111/j.1365-3040.2008.01902.x.
The heat stress response is universal to all organisms. Upon elevated temperatures, heat stress transcription factors (Hsfs) are activated to up-regulate the expression of molecular chaperones to protect cells against heat damages. In higher plants, the phenomenon is unusually complex both at the level of Hsfs and heat stress proteins (Hsps). Over-expression of both Hsfs and Hsps and the use of RNA interference for gene knock-down in a transient system in tomato protoplasts allowed us to dissect the in vivo chaperone functions of essential components of thermotolerance, such as the cytoplasmic sHsp, Hsp70 and Hsp100 chaperone families, and the regulation of their expression. The results point to specific functions of the different components in protection from protein denaturation and in refolding of denatured proteins.
热应激反应在所有生物体中都是普遍存在的。在温度升高时，热应激转录因子（Hsfs）被激活，以上调分子伴侣的表达，从而保护细胞免受热损伤。在高等植物中，这种现象在Hsfs和热应激蛋白（Hsps）水平上异常复杂。在番茄原生质体的瞬时系统中，Hsfs和Hsps的过表达以及利用RNA干扰进行基因敲除，使我们能够剖析耐热性关键成分的体内伴侣功能，例如细胞质小分子热激蛋白（sHsp）、Hsp70和Hsp100伴侣家族，以及它们表达的调控。结果表明不同成分在防止蛋白质变性和变性蛋白质重折叠方面具有特定功能。
