Orlandelli Ravely Casarotti, Vasconcelos Ana Flora Dalberto, Azevedo João Lúcio, Corradi da Silva Maria de Lourdes, Pamphile João Alencar
Universidade Estadual de Maringá, Departamento de Biotecnologia, Genética e Biologia Celular, CEP 87020-900, Maringá, Paraná, Brazil.
Universidade Estadual Paulista, Faculdade de Ciências e Tecnologia, Departamento de Química e Bioquímica, CEP 19060-900, Presidente Prudente, São Paulo, Brazil.
Biochim Open. 2016 Mar 2;2:33-40. doi: 10.1016/j.biopen.2016.02.003. eCollection 2016 Jun.
Endophytic fungi have been described as producers of important bioactive compounds; however, they remain under-exploited as exopolysaccharides (EPS) sources. Therefore, this work reports on EPS production by submerged cultures of eight endophytes isolated from Sw., belonging to genera , , , , and . After fermentation for 96 h, four endophytes secreted EPS: sp. JF767000, sp. JF766998, sp. JF767007 and JF766995. The EPS from sp. JF766998 differed statistically from the others, with a higher percentage of carbohydrate (91%) and lower amount of protein (8%). Subsequently, this fungus was grown under submerged culture for 72, 96 and 168 h (these EPS were designated EPS, EPS and EPS) and the differences in production, monosaccharide composition and apparent molecular were compared. The EPS yields in mg/100 mL of culture medium were: 3.0 ± 0.4 (EPS), 15.4 ± 2.2 (EPS) and 14.8 ± 1.8 (EPS). The EPS had high protein content (28.5%) and only 71% of carbohydrate; while EPS and EPS were composed mainly of carbohydrate (≈95 and 100%, respectively), with low protein content (≈5%) detected at 96 h. Galactose was the main monosaccharide component (30%) of EPS. Differently, EPS was rich in glucose (51%), with molecular weight of 46.6 kDa. It is an important feature for future investigations, because glucan-rich EPS are reported as effective antitumor agents.
内生真菌被认为是重要生物活性化合物的生产者；然而，作为胞外多糖（EPS）来源，它们仍未得到充分开发。因此，本研究报道了从Sw.分离出的8种内生真菌的液体培养物产生EPS的情况，这些内生真菌分别属于、、、、和属。发酵96小时后，4种内生真菌分泌了EPS：JF767000菌株、JF766998菌株、JF767007菌株和JF766995。来自JF766998菌株的EPS在统计学上与其他菌株不同，其碳水化合物百分比更高（91%），蛋白质含量更低（8%）。随后，将该真菌进行液体培养72、96和168小时（这些EPS分别命名为EPS、EPS和EPS），并比较了它们在产量、单糖组成和表观分子量方面的差异。每100毫升培养基中EPS的产量分别为：3.0±0.4（EPS）、15.4±2.2（EPS）和14.8±1.8（EPS）。EPS的蛋白质含量高（28.5%），碳水化合物含量仅为71%；而EPS和EPS主要由碳水化合物组成（分别约为95%和100%），在96小时时检测到的蛋白质含量较低（约5%）。半乳糖是EPS的主要单糖成分（30%）。不同的是，EPS富含葡萄糖（51%），分子量为46.6 kDa。这是未来研究的一个重要特征，因为富含葡聚糖的EPS被报道为有效的抗肿瘤剂。
