Dutta Swarnalee, Woo E-Eum, Yu Sang-Mi, Nagendran Rajalingam, Yun Bong-Sik, Lee Yong Hoon
Division of Biotechnology, Chonbuk National University, Iksan-si, Jeollabuk-do, Republic of Korea.
Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resources, Sangju-si, Gyeongsangbuk-do, Republic of Korea.
Mycobiology. 2019 Mar 23;47(1):87-96. doi: 10.1080/12298093.2018.1551833. eCollection 2019 Mar.
Fungi produce various secondary metabolites that have beneficial and harmful effects on other organisms. Those bioactive metabolites have been explored as potential medicinal and antimicrobial resources. However, the activities of the culture filtrate (CF) and metabolites of white-rot fungus () have been underexplored. In this study, we assayed the antimicrobial activities of CF obtained from white-rot fungus against various plant pathogens and evaluated its efficacy for controlling anthracnose and gray mold in pepper plants. The CF inhibited the mycelial growth of various fungal plant pathogens, but not of bacterial pathogens. Diluted concentrations of CF significantly suppressed the severity of anthracnose and gray mold in pepper fruits. Furthermore, the incidence of anthracnose in field conditions was reduced by treatment with a 12.5% dilution of CF. The active compound responsible for the antifungal and disease control activity was identified and verified as schizostatin. Our results indicate that the CF of white-rot fungus can be used as an eco-friendly natural product against fungal plant pathogens. Moreover, the compound, schizostatin could be used as a biochemical resource or precursor for development as a pesticide. To the best of our knowledge, this is the first report on the control of plant diseases using CF and active compound from white-rot fungus. We discussed the controversial antagonistic activity of schizostatin and believe that the CF of white-rot fungus or its active compound, schizostatin, could be used as a biochemical pesticide against fungal diseases such as anthracnose and gray mold in many vegetables.
真菌产生的各种次生代谢产物对其他生物具有有益和有害的影响。这些生物活性代谢产物已被探索作为潜在的药用和抗菌资源。然而，白腐真菌的培养滤液（CF）和代谢产物的活性尚未得到充分研究。在本研究中，我们测定了从白腐真菌获得的CF对各种植物病原体的抗菌活性，并评估了其对辣椒植株炭疽病和灰霉病的防治效果。CF抑制了各种真菌植物病原体的菌丝生长，但对细菌病原体没有抑制作用。CF的稀释浓度显著抑制了辣椒果实中炭疽病和灰霉病的严重程度。此外，在田间条件下，用12.5%稀释的CF处理可降低炭疽病的发病率。负责抗真菌和病害防治活性的活性化合物被鉴定并确认为裂褶菌素。我们的结果表明，白腐真菌的CF可作为一种生态友好型天然产物用于防治真菌植物病原体。此外，裂褶菌素可作为一种生化资源或开发农药的前体。据我们所知，这是关于使用白腐真菌的CF和活性化合物防治植物病害的首次报道。我们讨论了裂褶菌素存在争议的拮抗活性，并认为白腐真菌的CF或其活性化合物裂褶菌素可作为一种生化农药，用于防治许多蔬菜中的炭疽病和灰霉病等真菌病害。
