Velez de la Calle J F
Laboratoire de Cytogénétique, Cytologie et Biologie de la Reproduction, CHU de Brest, France.
Fertil Steril. 1991 Oct;56(4):737-42. doi: 10.1016/s0015-0282(16)54608-1.
Improve the technique of human spermatozoa separation using Percoll density gradients.
We compared the spermatozoa separation obtained after density gradients from a physiological stock Percoll solution and a hyperosmotic stock Percoll solution.
Sperm samples were obtained from the Laboratory of the Biology of Reproduction at Brest, France, a public hospital.
PATIENTS, PARTICIPANTS: One hundred thirty-eight healthy donors.
None.
The resolution of the separation was assessed by the number of motile spermatozoa recovered after separation using density gradients.
The improvement of the resolution of separation is at least twofold greater with the use of hyperosmotic gradients than with that obtained by physiological gradients. Moreover, the best resolutions of separation were obtained from pathological sperm. Finally, the influence of buoyant density spermatozoa on the resolution of separation by this technique is clearly demonstrated.
With this modification, the technique of Percoll density gradients, used for the separation of human spermatozoa, is strongly advised especially in pathological cases.
改进使用Percoll密度梯度分离人精子的技术。
我们比较了从生理性储备Percoll溶液和高渗性储备Percoll溶液的密度梯度获得的精子分离效果。
精子样本取自法国布雷斯特一家公立医院的生殖生物学实验室。
患者、参与者：138名健康捐赠者。
无。
使用密度梯度分离后回收的活动精子数量评估分离效果。
使用高渗梯度时，分离效果的改善比生理性梯度至少高出两倍。此外，从病理性精子中获得了最佳分离效果。最后，明确证明了精子浮力密度对该技术分离效果的影响。
通过这种改进，强烈建议将用于分离人精子的Percoll密度梯度技术，尤其是在病理性病例中。
