Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China.
Molecules. 2019 Jan 10;24(2):233. doi: 10.3390/molecules24020233.
Wen Luo Yin (WLY), a well-known traditional Chinese medicine formulation, has been used as a complementary therapy for the treatment of rheumatoid arthritis in clinical settings. However, the chemical constituents of WLY remain unclear. In this study, a high-performance liquid chromatography coupled with tandem mass spectrometry method was established to separate and comprehensively identify the chemical constituents of WLY. The analytes were eluted with a mobile phase of acetonitrile and 0.1% aqueous acetic acid. Mass detection was performed in both positive and negative ion mode. The MS/MS fragmentation pathways were proposed for the identification of the components. A total of 42 compounds including sesquiterpenes, alkaloids, biflavonoids, polyacetylenes, phenylpropanoids and acetylenic phenols were identified unambiguously or tentatively according to their retention times and mass behavior with those of authentic standards or literature data. The identification and structural elucidation of chemical constituents may provide important information for quality control and pharmacological research of WLY.
文罗饮（WLY）是一种著名的中药方剂，已在临床中作为类风湿关节炎的辅助治疗药物使用。然而，其化学成分仍不清楚。本研究建立了一种高效液相色谱-串联质谱法，用于分离和全面鉴定 WLY 的化学成分。分析物采用乙腈和 0.1%乙酸水溶液作为流动相洗脱。质谱检测分别在正、负离子模式下进行。提出了 MS/MS 裂解途径以鉴定成分。根据保留时间和与对照品或文献数据的质量行为，共鉴定出 42 种化合物，包括倍半萜、生物碱、二氢黄酮、炔烃、苯丙素和炔基酚。化学成分的鉴定和结构解析可为 WLY 的质量控制和药理研究提供重要信息。
