Ding Youchao, Sun Cheng, Xu Xinhua
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, PR China.
Eur J Mass Spectrom (Chichester). 2009;15(6):705-13. doi: 10.1255/ejms.1032.
A method has been established by using liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI/MS/MS) for simultaneous separation and identification of nine carcinogenic dyes (Acid Red 26, Direct Blue 6, Direct Black 38, Direct Red 28, Basic Red 9, Basic Violet 14, Disperse Blue 1, Disperse Orange 11 and Disperse Yellow 3) prohibited in textile materials under EU 2002/371/EC decision. Three selected reaction monitoring (SRM) transitions and negative/positive ion switching mode in one single analysis was applied to differentiate between the dyes of different classes. A lower limit of detection was achieved at 0.025-0.25 mg kg(-1) of positive ESI dyes and 0.005-0.025 mg kg(-1) of negative ESI dyes. The accurate and sensitive identification of the nine dissimilar analytes was achieved by combining the characterized ions and retention time of the standards, for example, the characterized ions of M - xNa, M -Cl and M + H, respectively were detected for the the sulfonated (acid and direct), basic and disperse dyes. The chromophore groups of azo, triphenylmethane and anthraquinone in precursor ions were found to have fragmented in SRM mode. These results demonstrated a sensitive, accurate and rapid identification.
采用液相色谱/电喷雾电离串联质谱法（LC/ESI/MS/MS）建立了一种方法，用于同时分离和鉴定欧盟2002/371/EC指令中禁止用于纺织材料的9种致癌染料（酸性红26、直接蓝6、直接黑38、直接红28、碱性红9、碱性紫14、分散蓝1、分散橙11和分散黄3）。在一次分析中应用三个选择反应监测（SRM）转换和负离子/正离子切换模式来区分不同类别的染料。正电喷雾电离（ESI）染料的检测下限为0.025 - 0.25 mg kg⁻¹，负电喷雾电离染料的检测下限为0.005 - 0.025 mg kg⁻¹。通过结合标准品的特征离子和保留时间，实现了对9种不同分析物的准确灵敏鉴定，例如，分别检测到了磺化（酸性和直接）、碱性和分散染料的特征离子M - xNa、M - Cl和M + H。发现前体离子中偶氮、三苯甲烷和蒽醌的发色团在SRM模式下发生了裂解。这些结果表明该方法具有灵敏、准确和快速的鉴定能力。
