夏枯草高效液相色谱指纹图谱研究
[Study on HPLC fingerprint of Prunella vulgaris].
作者信息
Sun Wei-Guang, Ke Xue-Hong, Li Yuan, Hua Ru-Feng, Chen Jin-Fu
机构信息
Guangzhou Xingqun Pharmaceutical Ltd. Co., Guangzhou 510288, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Sep;33(18):2090-3.
OBJECTIVE
To establish HPLC fingerprint of Prunella vulgarise for quality control of the herbal medicine.
METHOD
A sunfire C18 analytical column was used. The mobile phase A was 1% acetic acid, and mobile phase B was methanol. The elution was in gradient mode and detection wavelength was set at 290 nm. The flow rate was 1.0 mL x min(-1) and the column temperature at 30 degrees C. The analysis time was 60 min.
RESULT
The similarity of 10 batches of P. vulgaris was not lower than 0.810. The fingerprints of the herbal medicine were classified P. vulgaris on the results of cluster analysis.
CONCLUSION
This method is available for quality evaluation and control the quality of P. vulgaris.
目的
建立夏枯草的高效液相色谱指纹图谱，用于该中药材的质量控制。
方法
采用Sunfire C18分析柱。流动相A为1%乙酸，流动相B为甲醇。洗脱采用梯度洗脱模式，检测波长设定为290 nm。流速为1.0 mL·min⁻¹，柱温为30℃。分析时间为60 min。
结果
10批夏枯草的相似度不低于0.810。根据聚类分析结果，该中药材的指纹图谱可分为夏枯草类。
结论
该方法可用于夏枯草的质量评价和质量控制。
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