[络石藤的高效液相色谱指纹图谱研究]

[Study on HPLC fingerprint of Trachelospermum jasminoides].

作者信息

Liu Yiquan, Yu Nengjiang, Yang Xuedong, Zhao Yimin

机构信息

School of Pharmaceutical and Technology, Tianjin University, Tianjin 300072, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Mar;34(6):727-30.

PMID:19624016

Abstract

OBJECTIVE

To establish HPLC fingerprint for identification and evaluation of the quality of Trachelospermum jasminoides.

METHOD

The analysis was performed on a ZORBAX Eclipse XDB-C18 analytical column (4.6 mm x 150 mm, 5 microm) with H2O (A) and methanol (B) as mobile phases in gradient mode. The elutin conditions were 0-15 min, changed from 10% B to 30% B; 15-40 min, to 40% B; 40-60 min, to 60% B. The column temperature was set at 30 degrees C and the flow rate was 0.8 mL x min(-1) with the detection wavelength of 230 nm.

RESULT

The HPLC chromatographic fingerprint of T. jasminoides, showing 19 characteristic peaks, was established from 14 lots of T. jasminoides. The similarity of every lot of T. jasminoides was calculated as good in general, the similarity of 10 lots was above 0.9 and the other 4 lots had low similarity.

CONCLUSION

The chromatographic fingerprint of T. jasminoides with high characteristics and specificity can be used to control its quality.

摘要

目的

建立络石藤的高效液相色谱指纹图谱，用于其鉴别和质量评价。

方法

采用ZORBAX Eclipse XDB-C18分析柱（4.6 mm×150 mm，5μm），以水（A）和甲醇（B）为流动相，梯度洗脱。洗脱条件为：0 - 15分钟，B由10%变为30%；15 - 40分钟，变为40% B；40 - 60分钟，变为60% B。柱温设定为30℃，流速为0.8 mL·min⁻¹，检测波长为230 nm。