一种基于罗丹明异羟肟酸的次氯酸荧光探针及其在生物成像中的应用。
A rhodamine-hydroxamic acid-based fluorescent probe for hypochlorous acid and its applications to biological imagings.
作者信息
Yang Young-Keun, Cho Hyungseoph Jason, Lee Jihyun, Shin Injae, Tae Jinsung
机构信息
Department of Chemistry, Yonsei University, Seoul 120-749, Korea.
出版信息
Org Lett. 2009 Feb 19;11(4):859-61. doi: 10.1021/ol802822t.
A new rhodamine-hydroxamic acid-based fluorescent chemosensor for the rapid detection of HOCl in aqueous media was developed. The system, which utilizes an irreversible HOCl-promoted oxidation reaction, responds instantaneously at room temperature with linear proportionality to the amount of HOCl. This system is highly selective for HOCl over other reactive oxygen species (ROS) and highly sensitive in aqueous solutions. Biological imaging studies using living cells and organisms (A549 cells and zebrafish) to detect HOCl are successfully demonstrated.
开发了一种基于罗丹明异羟肟酸的新型荧光化学传感器，用于在水性介质中快速检测次氯酸。该系统利用不可逆的次氯酸促进氧化反应，在室温下即时响应，与次氯酸的量呈线性比例关系。该系统对次氯酸的选择性高于其他活性氧（ROS），并且在水溶液中具有高灵敏度。成功展示了使用活细胞和生物体（A549细胞和斑马鱼）进行检测次氯酸的生物成像研究。
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