Sun Zhen-Ning, Liu Feng-Qin, Chen Yan, Tam Paul Kwong Hang, Yang Dan
Department of Chemistry, The University of Hong Kong, Hong Kong, P.R. China.
Org Lett. 2008 Jun 5;10(11):2171-4. doi: 10.1021/ol800507m. Epub 2008 May 1.
A fluorescent probe, HKOCl-1, has been successfully developed for the detection of hypochlorous acid on the basis of a specific reaction with p-methoxyphenol. The formation of HOCl has been successfully detected not only in an abiotic system but also in an enzymatic system (myeloperoxidase/H2O2/Cl(-) system) and in living macrophage cells upon stimulation. This new probe might be used as an efficient tool for probing the roles HOCl plays in biological systems.
一种荧光探针HKOCl-1已成功研发出来，用于基于与对甲氧基苯酚的特定反应来检测次氯酸。不仅在非生物体系中，而且在酶体系（髓过氧化物酶/H2O2/Cl(-)体系）以及受刺激的活巨噬细胞中，都已成功检测到了次氯酸的形成。这种新型探针可能会成为探究次氯酸在生物体系中所起作用的有效工具。
