Zhang Kenneth Yin, Zhang Jie, Liu Yahong, Liu Shujuan, Zhang Pinglin, Zhao Qiang, Tang Yan, Huang Wei
Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials , Nanjing University of Posts and Telecommunications , Nanjing 210023 , P.R. China . Email:
Institute of Advanced Materials , Jiangsu-Singapore Joint Research Centre for Organic/Bio-Electronics & Information Displays , Nanjing Tech University , Nanjing 211816 , P.R. China.
Chem Sci. 2015 Jan 1;6(1):301-307. doi: 10.1039/c4sc02600d. Epub 2014 Oct 10.
We report a ratiometric phosphorescence sensory system for hypochlorite (ClO) based on core-shell structured silica nanoparticles. Two phosphorescent iridium(iii) complexes were immobilised in the inner solid core and outer mesoporous layer of the nanoparticles, respectively. The former is insensitive to ClO and thus serves as an internal standard to increase the accuracy and precision, while the latter exhibits a specific and significant luminogenic response to ClO, providing high selectivity and sensitivity. Upon exposure to ClO, the nanoparticles display a sharp luminescence colour change from blue to red. Additionally, intracellular detection of exogenous and endogenous ClO has been demonstrated ratiometric imaging and photoluminescence lifetime imaging microscopy. Compared to intensity-based sensing, ratiometric and lifetime-based measurements are independent of the probe concentration and are thus less affected by external influences, especially in intracellular applications.
我们报道了一种基于核壳结构二氧化硅纳米颗粒的用于次氯酸盐（ClO）的比率磷光传感系统。两种磷光铱（III）配合物分别固定在纳米颗粒的内部固体核和外部介孔层中。前者对ClO不敏感，因此用作内标以提高准确性和精密度，而后者对ClO表现出特异性且显著的发光响应，具有高选择性和灵敏度。暴露于ClO时，纳米颗粒显示出从蓝色到红色的急剧发光颜色变化。此外，已通过比率成像和光致发光寿命成像显微镜证明了对外源和内源性ClO的细胞内检测。与基于强度的传感相比，基于比率和寿命的测量与探针浓度无关，因此受外部影响较小，尤其是在细胞内应用中。
