伯氏疏螺旋体脑脊液分离株的初步鉴定

Preliminary characterization of Borrelia burgdorferi CSF isolates.

作者信息

Khanakah G, Millner M M, Müllegger R R, Stanek G

机构信息

Hygiene-Institut der Universität Wien, Austria.

出版信息

Infection. 1991 Jul-Aug;19(4):287-8. doi: 10.1007/BF01644969.

DOI:10.1007/BF01644969

PMID:1917048

Abstract

Borrelia burgdorferi was cultivated from three cerebrospinal fluid (CSF) samples of children (aged three and a half, four and a half and eight years) who were admitted to the hospital because of acute facial palsy, aseptic meningitis, and aseptic meningitis plus facial palsy. CSF was taken on day one in two cases and on day two in the remaining case after onset of symptoms. All three strains showed a very similar SDS-PAGE pattern, without an OspB and 20kD band. However, of nine monoclonal antibodies (Moab) raised against B. burgdorferi B31, the Moab H5332 recognized two strains, one of them very weakly, and the flagella specific Moabs H9724, H605, and H6TS (less intensively) recognized all strains. This preliminary characterization reveals heterogeneity among CSF Borrelia isolates of cases from a very close geographic area.

摘要

从三名因急性面瘫、无菌性脑膜炎以及无菌性脑膜炎合并面瘫入院的儿童（年龄分别为三岁半、四岁半和八岁）的三份脑脊液样本中培养出了伯氏疏螺旋体。在症状出现后的第一天采集了两份脑脊液样本，另一份在第二天采集。所有三株菌株均呈现出非常相似的SDS - PAGE图谱，没有OspB和20kD条带。然而，在针对伯氏疏螺旋体B31产生的九种单克隆抗体（Moab）中，单克隆抗体H5332识别出两株菌株，其中一株识别较弱，而鞭毛特异性单克隆抗体H9724、H605和H6TS（识别强度较弱）识别出所有菌株。这一初步特征揭示了来自非常相近地理区域的病例的脑脊液伯氏疏螺旋体分离株之间存在异质性。