Barbour A G, Hayes S F, Heiland R A, Schrumpf M E, Tessier S L
Infect Immun. 1986 May;52(2):549-54. doi: 10.1128/iai.52.2.549-554.1986.
In immunofluorescence assays monoclonal antibody H9724 recognized eight species of the spirochetal genus Borrelia but not representatives of the genera Treponema, Leptospira, and Spirochaeta. We examined the reactivity of H9724 against subcellular components of Borrelia hermsii, an agent of relapsing fever, and B. burgdorferi, the cause of Lyme disease. H9724 bound to isolated periplasmic flagella of the two borreliae. In Western blots the antibody reacted with the predominant protein in flagellar preparations from B. hermsii and B. burgdorferi; the apparent molecular weights of these flagellins were 39,000 and 41,000, respectively.
在免疫荧光试验中，单克隆抗体H9724可识别疏螺旋体属的8个种，但不能识别密螺旋体属、钩端螺旋体属和螺旋体属的代表菌株。我们检测了H9724对回归热病原体赫氏疏螺旋体和莱姆病病原体伯氏疏螺旋体亚细胞成分的反应性。H9724与分离出的这两种疏螺旋体的周质鞭毛结合。在蛋白质印迹法中，该抗体与赫氏疏螺旋体和伯氏疏螺旋体鞭毛制剂中的主要蛋白质发生反应；这些鞭毛蛋白的表观分子量分别为39,000和41,000。
