Okiji T, Kawashima N, Kosaka T, Kobayashi C, Suda H
Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
J Endod. 1994 Jan;20(1):27-31. doi: 10.1016/s0099-2399(06)80023-3.
Periapical lesions were experimentally produced in rat lower first molars by exposing the pulp to the oral environment for 1 to 56 days. Temporal changes in the number and distribution of la antigen-expressing nonlymphoid cells in the periapical tissue were examined immunohistochemically on decalcified cryostat sections using OX6, a monoclonal antibody against rat la antigen. Influx of la-positive exudative cells into the periapical tissue was observed from 1 day postoperatively. Between 14 and 28 days when expansion of the periapical lesion was most evident, numerous la-positive macrophage-like and dendritic cell-like cells of diverse morphologies were found in the periapical tissue. The number of these cells showed further increase at 56 days postoperatively, when the lesion expansion had ceased. These results suggest the involvement of la antigen-expressing nonlymphoid cells in the development and perpetuation of periapical pathosis. They may act primarily as antigen-presenting cells, which are essential for the initiation of antigen-specific immune defense.
通过将大鼠下颌第一磨牙的牙髓暴露于口腔环境1至56天，在实验中制造根尖周病变。使用抗大鼠Ia抗原的单克隆抗体OX6，通过免疫组织化学方法在脱钙低温恒温切片上检查根尖周组织中表达Ia抗原的非淋巴细胞的数量和分布的时间变化。术后1天观察到Ia阳性渗出细胞流入根尖周组织。在术后14至28天，当根尖周病变扩展最为明显时，在根尖周组织中发现了许多形态各异的Ia阳性巨噬细胞样和树突状细胞样细胞。术后56天，当病变扩展停止时，这些细胞的数量进一步增加。这些结果表明表达Ia抗原的非淋巴细胞参与了根尖周病变的发生和持续。它们可能主要作为抗原呈递细胞，这对抗原特异性免疫防御的启动至关重要。
