Aarli A, Matre R, Thunold S
Broegelmann Research Laboratory for Microbiology, Gade Institute, University of Bergen, Norway.
Int Arch Allergy Appl Immunol. 1991;95(1):64-9. doi: 10.1159/000235456.
IgG Fc receptors (FcR) in cryostat sections of human kidney were studied using functional assays and monoclonal antibodies (MoAbs). Using functional assays, FcR were detected on cells in glomeruli and distal convoluted tubuli, on endothelial cells and on interstitial cells. These cells were also stained with B1D6, a MoAb reactive with a 40-kD molecule with low affinity FcR activity. Using MoAbs against leukocyte FcR, a different pattern of reactivity was obtained. MoAbs against FcR I (32.2), FcR II (IV.3 and C1KM5) and FcR III (3G8 and Leu11b) stained interstitial macrophages only. The data confirm the presence of FcR on renal glomeruli and interstitial cells and also indicate that epithelial cells of distal tubuli and endothelial cells express FcR. Furthermore, renal FcR appear to be structurally different from the FcR I, II and III defined on leukocytes.
利用功能分析和单克隆抗体（MoAb）对人肾低温切片中的IgG Fc受体（FcR）进行了研究。通过功能分析，在肾小球、远曲小管的细胞、内皮细胞和间质细胞上检测到了FcR。这些细胞也被B1D6染色，B1D6是一种与具有低亲和力FcR活性的40-kD分子反应的单克隆抗体。使用针对白细胞FcR的单克隆抗体，获得了不同的反应模式。针对FcR I（32.2）、FcR II（IV.3和C1KM5）和FcR III（3G8和Leu11b）的单克隆抗体仅对间质巨噬细胞进行染色。数据证实了肾小球和间质细胞上存在FcR，也表明远曲小管的上皮细胞和内皮细胞表达FcR。此外，肾FcR在结构上似乎与在白细胞上定义的FcR I、II和III不同。
