An H, Scopes R K, Rodriguez M, Keshav K F, Ingram L O
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
J Bacteriol. 1991 Oct;173(19):5975-82. doi: 10.1128/jb.173.19.5975-5982.1991.
The 13 major enzymes which compose the glycolytic and fermentative pathways in Zymomonas mobilis are particularly abundant and represent one-half of the soluble protein in exponential-phase cells. One- and two-dimensional polyacrylamide gel electrophoresis maps were developed for 12 of these enzymes. Assignments were made by comigration with purified proteins, comparison with overexpressed genes in recombinant strains, and Western blots (immunoblots). Although most glycolytic enzymes appeared resistant to turnover and accumulated in stationary-phase cells, the protein levels of pyruvate kinase, alcohol dehydrogenase I, and glucokinase declined. Alcohol dehydrogenase II was identified as a major stress protein and was induced both by exposure to ethanol and by elevated temperature (45 degrees C). This enzyme, encoded by the adhB gene, is expressed from tandem promoters which share partial sequence identity with the Escherichia coli consensus sequence for heat shock proteins.
运动发酵单胞菌中构成糖酵解和发酵途径的13种主要酶特别丰富，占对数期细胞中可溶性蛋白质的一半。针对其中12种酶绘制了一维和二维聚丙烯酰胺凝胶电泳图谱。通过与纯化蛋白质共迁移、与重组菌株中过表达基因比较以及蛋白质免疫印迹法（免疫印迹）进行鉴定。尽管大多数糖酵解酶似乎不易周转并在稳定期细胞中积累，但丙酮酸激酶、乙醇脱氢酶I和葡萄糖激酶的蛋白质水平下降。乙醇脱氢酶II被鉴定为一种主要应激蛋白，在暴露于乙醇和温度升高（45摄氏度）时均被诱导。这种由adhB基因编码的酶由串联启动子表达，这些启动子与大肠杆菌热休克蛋白的共有序列具有部分序列同一性。
